Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-BMP4 antibody

Inconclusive
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Xenopus laevis Tissue sections (NF stage embryos)
Antigen retrieval step
Heat mediated
Permeabilization
Yes - PBT (0.1% Triton in PBS)
Specification
NF stage embryos
Blocking step
(agent) for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Formaldehyde

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% neutral goat serum in PBT
Dilution
1/625

Secondary antibody

Dilution
1/200
Name
Non-Abcam antibody was used: Vector peroxidase anti-rabbit IgG
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase

Additional data

Additional Notes
I did immunohistochemistry on a NF 41 stage head skeleton of Xenopus tadpole and got no staining at all.
Abcam response
Thank you for submitting this review as part of our AbTrial Testing Program. Your results suggest that the antibody may not react with Xenopus laevis. We welcome more feedback for this species.

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Submitted Dec 07 2015

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