For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Xenopus laevis Tissue sections (NF stage embryos)
Antigen retrieval step
Yes - PBT (0.1% Triton in PBS)
NF stage embryos
(agent) for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Other product details
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% neutral goat serum in PBT
Non-Abcam antibody was used: Vector peroxidase anti-rabbit IgG
Host species: Goat
Conjugation: Horse Radish Peroxidase
I did immunohistochemistry on a NF 41 stage head skeleton of Xenopus tadpole and got no staining at all.
Thank you for submitting this review as part of our AbTrial Testing Program. Your results suggest that the antibody may not react with Xenopus laevis. We welcome more feedback for this species.
Abcam user community
Submitted Dec 07 2015