Immunohistochemistry (Frozen sections) abreview for Anti-BMP4 antibody

Inconclusive
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
Yes - Triton X-100
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s)
Dilution
1/500

Secondary antibody

Dilution
1/500
Name
Non-Abcam antibody was used: anti-rabbit Alexa Fluor 555 conjugated
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555

Additional data

Additional Notes
Tested conditions: 1) 1:250 1:500 1:1000 2) 1:250, 1:500, 1:1000 with streptavidin enhancement 3) 1:500 incubation of 1 Ab at 37C for 5h 4) 1:250 1:500 1:1000 1Ab at 37C for 5h and streptavidin enhancement Dilutions refer to primary antibody. We were not able to get signal above background.
Abcam response
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.

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Submitted Sep 28 2016

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