Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6211] to BMP4 (Biotin)
- Suitable for: IHC-P
- Reacts with: Human
- Conjugation: Biotin
Product nameAnti-BMP4 antibody [EPR6211] (Biotin)
See all BMP4 primary antibodies
DescriptionRabbit monoclonal [EPR6211] to BMP4 (Biotin)
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human BMP4 aa 350 to the C-terminus. The exact sequence is proprietary.
- IHC-P: FFPE, normal human colon tissue.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Dissociation constant (KD)KD = 1.94 x 10 -10 M Learn more about KD
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
- Anti-BMP4 antibody [EPR6211] (ab124715)
- Anti-BMP4 antibody [EPR6211] (Alexa Fluor® 488) (ab200794)
- Anti-BMP4 antibody [EPR6211] (HRP) (ab200795)
- Anti-BMP4 antibody [EPR6211] (Alexa Fluor® 647) (ab200796)
- Anti-BMP4 antibody [EPR6211] (Alexa Fluor® 594) (ab202526)
- Anti-BMP4 antibody [EPR6211] (Alexa Fluor® 568) (ab202527)
- Anti-BMP4 antibody [EPR6211] - Low endotoxin, Azide free (ab216455)
Our Abpromise guarantee covers the use of ab202525 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionInduces cartilage and bone formation. Also act in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction.
Tissue specificityExpressed in the lung and lower levels seen in the kidney. Present also in normal and neoplastic prostate tissues, and prostate cancer cell lines.
Involvement in diseaseDefects in BMP4 are the cause of microphthalmia syndromic type 6 (MCOPS6) [MIM:607932]; also known as microphthalmia and pituitary anomalies or microphthalmia with brain and digit developmental anomalies. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS6 is characterized by microphthalmia/anophthalmia associated with facial, genital, skeletal, neurologic and endocrine anomalies.
Defects in BMP4 are the cause of non-syndromic orofacial cleft type 11 (OFC11) [MIM:600625]. Non-syndromic orofacial cleft is a common birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum. OFC11 is an unusual anomaly consisting of a paramedian scar of the upper lip with an appearance suggesting that a typical cleft lip was corrected in utero.
Sequence similaritiesBelongs to the TGF-beta family.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- zgc:100779 antibody
- BMP 2B antibody
- BMP 4 antibody
IHC image of BMP4 staining in a section of formalin-fixed paraffin-embedded normal human colon*, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins, before blocking of endogenous biotin. The section was then incubated with 202525, 1/100 dilution, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
ab202525 has not yet been referenced specifically in any publications.