Product nameAnti-BMP6 antibody [morph-6.1]
See all BMP6 primary antibodies
DescriptionMouse monoclonal [morph-6.1] to BMP6
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Rat, Dog, Human
- Positive staining can be observed in embryonic and adult central nervous system. Rat radial glial cells of the developing central nervous system from E11 to E19. In rat peripheral nerves a selective intracellular immunoreactivity can be found in perinuclear region of most Schwann cells which form the myelin sheath. However, some Schwann cells were negative for the antigen.
Pre-incubation of anti BMP6 with amino-terminal peptide 1-29 of BMP6 inhibits binding of the mAb to tissue sections. Pre-incubation with other amino-terminal synthetic peptides of human BMP7, BMP4, BMP3 and BMP2 as well as an irrelevant peptide does not inhibit specific tissue staining.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
Concentration information loading...
Primary antibody notesPre-incubation of anti BMP6 with amino-terminal peptide 1-29 of BMP6 inhibits binding of the mAb to tissue sections. Pre-incubation with other amino-terminal synthetic peptides of human BMP7, BMP4, BMP3 and BMP2 as well as an irrelevant peptide does not inhibit specific tissue staining.
Our Abpromise guarantee covers the use of ab15640 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||1/100. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).|
FunctionInduces cartilage and bone formation.
Sequence similaritiesBelongs to the TGF-beta family.
- Information by UniProt
- BMP-6 antibody
- Bmp6 antibody
- BMP6_HUMAN antibody
ab15640 staining human U2-OS cells by ICC/IF. Cells were PFA fixed and permeabilized in Triton-X-100 prior to blocking in 2% serum for 1 hour at 20°C. The primary antibody was diluted 1/100 and incubated with the sample for 1 hour at 37°C. The secondary antibody was Alexa Fluor®488 conjugated chicken anti-mouse, diluted 1/1000.
All lanes : Anti-BMP6 antibody [morph-6.1] (ab15640) at 1/400 dilution
All lanes : mouse liver
Lysates/proteins at 20 µg per lane.
All lanes : Non-Abcam Antibody was used: Dako Cytomation P0260
Conjugation: HRP at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 57 kDa
Additional bands at: 25&30&75 kDa (possible non-specific binding)
Exposure time: 10 seconds
ICC/IF image of ab15640 staining primary myoblast cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 5% normal donkey serum / 0.3M glycine in 0.1% PBS-Tritonx100 for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15640, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488donkey anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h.
This product has been referenced in:
- Garrison P et al. Spatial regulation of bone morphogenetic proteins (BMPs) in postnatal articular and growth plate cartilage. PLoS One 12:e0176752 (2017). IHC ; Mouse . Read more (PubMed: 28467498) »
- Díaz PU et al. BMP2, 4 and 6 and BMPR1B are altered from early stages of bovine cystic ovarian disease development. Reproduction 152:333-50 (2016). Cow . Read more (PubMed: 27486268) »