Western blot abreview for Anti-BMP6 antibody [morph-6.1]

Good
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Loading amount
20 µg
Specification
Liver
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 4°C

Other product details

Dilution
1/400
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: TBST plus 1% milk

Secondary antibody

Name
Non-Abcam antibody was used: Dako Cytomation P0260
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/3000

Detection

Detection method
ECL+
Exposure
10 second(s)
Bands
Specific: 57 kDa Non-specific: 25, 30, 75 kDa
Positive control
Liver
Negative control
None

Additional data

Additional Notes
Lane 1: Adult Liver (Mouse) Tissue Lysate
Lane 2: Adult Liver (Mouse) Tissue Lysate
Lane 3: Adult Liver (Mouse) Tissue Lysate
Lane 4: Adult Liver (Mouse) Tissue Lysate
Abcam response
Many thanks for your data in WB for ab15640 (BMP6 antibody [morph-6.1]) on mouse samples.

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Jun 02 2009

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