Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Other product details
24 hour(s) and 0 minute(s) · Temperature: 4°C
Non-Abcam antibody was used: Alexa Fluor® 488donkey anti mouse Host species: Donkey Clonality: Monoclonal Conjugation: Alexa Fluor® 488
ICC/IF image of ab15640 staining primary myoblast cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 5% normal donkey serum / 0.3M glycine in 0.1% PBS-Tritonx100 for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15640, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488donkey anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h.
Dr. Ruma Raha-Chowdhury
Submitted Dec 14 2009
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