Western blot abreview for Anti-BMP6 antibody [morph-6.1]

Good
Abreviews
Application
Western blot
Sample
Rat Cell lysate - whole cell (Schwannom cell line RN 22)
Loading amount
25 µg
Specification
Schwannom cell line RN 22
Gel Running Conditions
Reduced Denaturing (15% SDS)
Blocking step
Odyssey Blocking Buffer 1:1 diluted with PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 20°C

Other product details

Dilution
1/100
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: Odyssey Blocking Buffer / PBS

Secondary antibody

Name
Non-Abcam antibody was used: IRDye 680 Goat Anti-Mouse IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 700DX
Dilution
1/8000

Detection

Detection method
Infrared scanning
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 57 kDa Non-specific: 40 kDa

Additional data

Additional Notes
Used PVDF-FL membrane

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Submitted Feb 19 2008

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