Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab27569 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.5 µg/ml.
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 49 kDa.
Sandwich ELISA Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to BMP7 (Biotin) (ab84030). Use as Capture antibody.
ICC/IF Use a concentration of 1 - 5 µg/ml.
Neutralising Use at an assay dependent dilution.

Target

  • Function
    Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis.
  • Tissue specificity
    Expressed in the kidney and bladder. Lower levels seen in the brain.
  • Sequence similarities
    Belongs to the TGF-beta family.
  • Developmental stage
    Expressed in the developing eye, brain and ear during embryogenesis.
  • Post-translational
    modifications
    Several N-termini starting at positions 293, 300, 315 and 316 have been identified by direct sequencing resulting in secretion of different mature forms (PubMed:17977014).
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • BMP 7 antibody
    • BMP-7 antibody
    • Bmp7 antibody
    • BMP7_HUMAN antibody
    • Bone morphogenetic protein 7 antibody
    • Bone morphogenic protein 7 antibody
    • Eptotermin alfa antibody
    • OP 1 antibody
    • OP-1 antibody
    • OP1 antibody
    • Osteogenic protein 1 antibody
    see all

Images

  • ICC/IF image of ab27569 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab27569, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ICC/IF image of Rat primary hepatocytes culture cells stained with ab27569. The cells were fixed with 4% PFA and incubated in  5% normal donkey serum in 0.1% PBS- and triton X100  for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab27569, 1µg/ml) and (ab15640) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 568 (red) at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Connolly NP  et al. Cross-species transcriptional analysis reveals conserved and host-specific neoplastic processes in mammalian glioma. Sci Rep 8:1180 (2018). Read more (PubMed: 29352201) »
  • Heinonen AM  et al. Neuroprotection by rAAV-mediated gene transfer of bone morphogenic protein 7. BMC Neurosci 15:38 (2014). WB ; Mouse . Read more (PubMed: 24618040) »
See all 4 Publications for this product

Customer reviews and Q&As

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1-3 of 3 Abreviews

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Bone marrow derived mast cells)
Permeabilization
Yes - 0.1% Triton-X 100
Specification
Bone marrow derived mast cells
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative
Paraformaldehyde

Mrs. Nicole Corder

Verified customer

Submitted Jul 30 2018

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Primary hepatocytes culture)
Specification
Primary hepatocytes culture
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% Tritox100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Dec 14 2009

Application
Western blot
Sample
Human Cell lysate - whole cell (OVCAR5 cell)
Loading amount
50 µg
Specification
OVCAR5 cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Mr. John Andersen

Verified customer

Submitted Jun 29 2006

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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