Overview

  • Product name
  • Description
    Rabbit polyclonal to BMP7
  • Host species
    Rabbit
  • Specificity
    This antibody reacts with BMP7.
  • Tested applications
    Suitable for: IHC-Fr, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide: QGKHNSAPMF MLDLYNAMAV EEGGGPGGQG FSYPYKAVFS TQGPPLASLQ, corresponding to N terminal amino acids 73-122 of Human BMP7. This sequence is derived from the propeptide domain, thus, will not recognize mature BMP7.

  • Positive control

Properties

Applications

Our Abpromise guarantee covers the use of ab56023 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).Can be blocked with Human BMP7 peptide (ab111668). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ICC/IF Use a concentration of 1 - 5 µg/ml.

Target

  • Function
    Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis.
  • Tissue specificity
    Expressed in the kidney and bladder. Lower levels seen in the brain.
  • Sequence similarities
    Belongs to the TGF-beta family.
  • Developmental stage
    Expressed in the developing eye, brain and ear during embryogenesis.
  • Post-translational
    modifications
    Several N-termini starting at positions 293, 300, 315 and 316 have been identified by direct sequencing resulting in secretion of different mature forms (PubMed:17977014).
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • BMP 7 antibody
    • BMP-7 antibody
    • Bmp7 antibody
    • BMP7_HUMAN antibody
    • Bone morphogenetic protein 7 antibody
    • Bone morphogenic protein 7 antibody
    • Eptotermin alfa antibody
    • OP 1 antibody
    • OP-1 antibody
    • OP1 antibody
    • Osteogenic protein 1 antibody
    see all

Images

  • Predicted band size: 49 kDa
    Observed band size: 49 kDa

  • Lane 1 : Anti-BMP7 antibody (ab56023) at 1.25 µg/ml
    Lane 2 : Anti-BMP7 antibody (ab56023) at 2.5 µg/ml
    Lane 3 : Anti-BMP7 antibody (ab56023) at 5 µg/ml

    All lanes : Cell lysate prepared from BMP7 transfected 293T cells

    Lysates/proteins at 25 µg per lane.

    Predicted band size: 49 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling BMP7 with ab56023 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

  • ICC/IF image of ab56023 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56023, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-BMP7 antibody (ab56023) at 1/500 dilution

    Lane 1 : Rat Brain Whole Tissue (age P8) Lysate
    Lane 2 : Rat Brain Whole Tissue (age P15) Lysate
    Lanes 3-4 : Rat Brain Whole Tissue (age P21) Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP conjugated at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 49 kDa
    Observed band size: 49 kDa


    Exposure time: 30 seconds


    A number of additional non-specific bands were also observed at 17, 33, 35 & 40kDa

    See Abreview

  • ab56023 staining mouse e18 skeleton tissues by immunohistochemistry (frozen sections). Sections were formaldehyde fixed prior to being blocked in 20% serum for 1 hour at RT. Sample was incubated with ab56023, diluted 1/1000, for 16 hours at 4°C. Alexa fluor® 680 donkey polyclonal, diluted 1/200, was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Sun Y  et al. BMP7 regulates lung fibroblast proliferation in newborn rats with bronchopulmonary dysplasia. Mol Med Rep 17:6277-6284 (2018). Read more (PubMed: 29512787) »
  • Wang L  et al. Decreased BMP-7 and p-Smad1/5/8 expression, and increased levels of gremlin in hepatocellular carcinoma. Oncol Lett 16:2113-2118 (2018). Read more (PubMed: 30008908) »
See all 17 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (9% SDS-PAGE)
Sample
Rat Tissue lysate - whole (Rat liver)
Specification
Rat liver
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 10 2014

Answer

Thank you for your enquiry.

All tested applications and species covered by our 6 month guarantee are specified on our datasheets, and these are updated as soon as any new information is brought to our attention.

Therefore, I can confirm that RANK antibody ab13918 is indeed tested and covered by the guarantee for both IHC-P and flow cytometry.

RUNX2 antibody ab102712
This is tested for IHC-P (paraffin embedded sections) as stated on the datasheet. However, I am sorry to confirm this has not been tested in flow cytometry.

The following RUNX2 antibody is tested in flow cytometry, I hope this may be of interest to your customer. However, please note this has not been tested in IHC and you will also need to check which species the customer is using. This has been tested and covered by the guarantee for human samples only.

ab135674 RUNX2 antibody
Tested in: Flow Cyt, WB
Reacts with: Human
https://www.abcam.com/index.html?datasheet=135674 (or use the following: https://www.abcam.com/index.html?datasheet=135674).


RANKL antibody ab45039
This is tested for IHC-P (paraffin embedded sections) as stated on the datasheet. However, I am sorry to confirm this has not been tested in flow cytometry.

We do have the following RANKL antibodies tested in flow cytometry. Again, please note that these are not tested in IHC and please also check the species the customer is using:

ab12125 RANKL antibody [12A380]
Tested in: Flow Cyt, WB
Reacts with: Human, Mouse
https://www.abcam.com/index.html?datasheet=12125 (or use the following: https://www.abcam.com/index.html?datasheet=12125).

ab95720 RANKL antibody [IK22/5] (Biotin)
Tested in: Flow Cyt
Reacts with: Mouse
https://www.abcam.com/index.html?datasheet=95720 (or use the following: https://www.abcam.com/index.html?datasheet=95720).

ab93553 RANKL antibody [IK22/5] (Phycoerythrin)
Tested in: Flow Cyt
Reacts with: Mouse
https://www.abcam.com/index.html?datasheet=93553 (or use the following: https://www.abcam.com/index.html?datasheet=93553).

BMP-7 antibody ab56023
This is tested for IHC-P (paraffin embedded sections). However, I am sorry to confirm this has not been tested in flow cytometry.

We do have the following BMP-7 antibody tested in flow cytometry. Again, please note that this is unfortunately not tested in IHC and please also check the species the customer is using:

ab84030 BMP7 antibody (Biotin)
Tested in: ELISA, Flow Cyt, sELISA, WB
Reacts with: Human, Chinese Hamster
https://www.abcam.com/index.html?datasheet=84030 (or use the following: https://www.abcam.com/index.html?datasheet=84030).

Osteoprotegerin (OPG) antibody ab73400
This is tested for IHC-P (paraffin embedded sections). However, I am sorry to confirm this has not been tested in flow cytometry.

The following Osteoprotegerin antibody is tested in both IHC-P and flow cytometry. Please also check the species the customer is using to ensure it is suitable:

ab105935 Osteoprotegerin antibody [ 5G2]
Tested in: ELISA, Flow Cyt, ICC, ICC/IF, IHC-P, WB
Reacts with: Human
https://www.abcam.com/index.html?datasheet=105935 (or use the following: https://www.abcam.com/index.html?datasheet=105935).

If your customer would like to test any of these antibodies in a previously untested species or application, please do not hesitate to contact us again prior to the purchase by replying to this message as you may be eligible for our testing discount program.

Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:

https://www.abcam.com/abreviews

I hope this information is helpful. Please do not hesitate to contact me for any further advice or information.

Read More

Answer

Thank you for contacting us. I have checked the image and can say that there are many unnecessary bands on the gel. I am happy to help you however I would need more information about the used protocol. Please answer my following questions;

What was the
- Order Number
- Lot number
- Storage temperature of ab
- memmory tumour cell lines
- amount of loaded protein
- Lysis buffer used? Was there any protease inhibitor in the lysis buffer?
- Dilution of antibody when used?

- Have you tried no primary control?
- Do you think multiple bands are not from primary antibody?
- At what temperature the lysates were heated with loading buffer?
- At what dilution the secondary was used? What was the secondary antibody?
- Did you use fresh lysates or frozen lysates?

Finally, do you have antibody left to try optimization suggestions.

Answers to these question will help me to understand where the problem was.

I will look forward o hearing from you soon.

Read More

Answer

Thank you for your inquiry. We do not assign strict expiration dates for our products, however we follow general guidelines: Antibodies stored at 4C may be viable up to 1 year, those stored at -20C may be viable for up to 5 years, and those stored at -80C may be viable for up to 10 years. I hope this information helps. Please contact us with any other questions.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Brain Lysate)
Loading amount
20 µg
Specification
Brain Lysate
Gel Running Conditions
Reduced Non-Denaturing (Native) (15% Gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Mar 24 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (e18 Skeleton)
Specification
e18 Skeleton
Fixative
Formaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Nov 04 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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