Overview

  • Product name
  • Description
    Rabbit polyclonal to BMP7
  • Host species
    Rabbit
  • Specificity
    This antibody reacts with BMP7.
  • Tested applications
    Suitable for: IHC-Fr, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide: QGKHNSAPMF MLDLYNAMAV EEGGGPGGQG FSYPYKAVFS TQGPPLASLQ, corresponding to N terminal amino acids 73-122 of Human BMP7. This sequence is derived from the propeptide domain, thus, will not recognize mature BMP7.

  • Positive control

Properties

Applications

Our Abpromise guarantee covers the use of ab56023 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).Can be blocked with Human BMP7 peptide (ab111668). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ICC/IF Use a concentration of 1 - 5 µg/ml.

Target

  • Function
    Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis.
  • Tissue specificity
    Expressed in the kidney and bladder. Lower levels seen in the brain.
  • Sequence similarities
    Belongs to the TGF-beta family.
  • Developmental stage
    Expressed in the developing eye, brain and ear during embryogenesis.
  • Post-translational
    modifications
    Several N-termini starting at positions 293, 300, 315 and 316 have been identified by direct sequencing resulting in secretion of different mature forms (PubMed:17977014).
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • BMP 7 antibody
    • BMP-7 antibody
    • Bmp7 antibody
    • BMP7_HUMAN antibody
    • Bone morphogenetic protein 7 antibody
    • Bone morphogenic protein 7 antibody
    • Eptotermin alfa antibody
    • OP 1 antibody
    • OP-1 antibody
    • OP1 antibody
    • Osteogenic protein 1 antibody
    see all

Images

  • Predicted band size: 49 kDa
    Observed band size: 49 kDa

  • Lane 1 : Anti-BMP7 antibody (ab56023) at 1.25 µg/ml
    Lane 2 : Anti-BMP7 antibody (ab56023) at 2.5 µg/ml
    Lane 3 : Anti-BMP7 antibody (ab56023) at 5 µg/ml

    All lanes : Cell lysate prepared from BMP7 transfected 293T cells

    Lysates/proteins at 25 µg per lane.

    Predicted band size: 49 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling BMP7 with ab56023 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

  • ICC/IF image of ab56023 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56023, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-BMP7 antibody (ab56023) at 1/500 dilution

    Lane 1 : Rat Brain Whole Tissue (age P8) Lysate
    Lane 2 : Rat Brain Whole Tissue (age P15) Lysate
    Lanes 3-4 : Rat Brain Whole Tissue (age P21) Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP conjugated at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 49 kDa
    Observed band size: 49 kDa


    Exposure time: 30 seconds


    A number of additional non-specific bands were also observed at 17, 33, 35 & 40kDa

    See Abreview

  • ab56023 staining mouse e18 skeleton tissues by immunohistochemistry (frozen sections). Sections were formaldehyde fixed prior to being blocked in 20% serum for 1 hour at RT. Sample was incubated with ab56023, diluted 1/1000, for 16 hours at 4°C. Alexa fluor® 680 donkey polyclonal, diluted 1/200, was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Miao N  et al. Loss of Fam20c causes defects in the acinar and duct structure of salivary glands in mice. Int J Mol Med 43:2103-2117 (2019). Read more (PubMed: 30864688) »
  • Kim Y  et al. Evaluation of Mesenchymal Stem Cell Sheets Overexpressing BMP-7 in Canine Critical-Sized Bone Defects. Int J Mol Sci 19:N/A (2018). Read more (PubMed: 30018197) »
See all 19 Publications for this product

Customer reviews and Q&As

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1-3 of 3 Abreviews

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (9% SDS-PAGE)
Sample
Rat Tissue lysate - whole (Rat liver)
Specification
Rat liver
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 10 2014

Application
Western blot
Sample
Rat Tissue lysate - whole (Brain Lysate)
Loading amount
20 µg
Specification
Brain Lysate
Gel Running Conditions
Reduced Non-Denaturing (Native) (15% Gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Mar 24 2010

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (e18 Skeleton)
Specification
e18 Skeleton
Fixative
Formaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Nov 04 2009

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