• Product name
  • Description
    Rabbit polyclonal to BMP7
  • Host species
  • Specificity
    This antibody reacts with BMP7.
  • Tested applications
    Suitable for: IHC-Fr, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide: QGKHNSAPMF MLDLYNAMAV EEGGGPGGQG FSYPYKAVFS TQGPPLASLQ, corresponding to N terminal amino acids 73-122 of Human BMP7. This sequence is derived from the propeptide domain, thus, will not recognize mature BMP7.

  • Positive control



Our Abpromise guarantee covers the use of ab56023 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).Can be blocked with Human BMP7 peptide (ab111668). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ICC/IF Use a concentration of 1 - 5 µg/ml.


  • Function
    Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis.
  • Tissue specificity
    Expressed in the kidney and bladder. Lower levels seen in the brain.
  • Sequence similarities
    Belongs to the TGF-beta family.
  • Developmental stage
    Expressed in the developing eye, brain and ear during embryogenesis.
  • Post-translational
    Several N-termini starting at positions 293, 300, 315 and 316 have been identified by direct sequencing resulting in secretion of different mature forms (PubMed:17977014).
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • BMP 7 antibody
    • BMP-7 antibody
    • Bmp7 antibody
    • BMP7_HUMAN antibody
    • Bone morphogenetic protein 7 antibody
    • Bone morphogenic protein 7 antibody
    • Eptotermin alfa antibody
    • OP 1 antibody
    • OP-1 antibody
    • OP1 antibody
    • Osteogenic protein 1 antibody
    see all


  • Predicted band size: 49 kDa
    Observed band size: 49 kDa

  • Lane 1 : Anti-BMP7 antibody (ab56023) at 1.25 µg/ml
    Lane 2 : Anti-BMP7 antibody (ab56023) at 2.5 µg/ml
    Lane 3 : Anti-BMP7 antibody (ab56023) at 5 µg/ml

    All lanes : Cell lysate prepared from BMP7 transfected 293T cells

    Lysates/proteins at 25 µg per lane.

    Predicted band size: 49 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling BMP7 with ab56023 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

  • ICC/IF image of ab56023 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56023, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-BMP7 antibody (ab56023) at 1/500 dilution

    Lane 1 : Rat Brain Whole Tissue (age P8) Lysate
    Lane 2 : Rat Brain Whole Tissue (age P15) Lysate
    Lanes 3-4 : Rat Brain Whole Tissue (age P21) Lysate

    Lysates/proteins at 20 µg per lane.

    All lanes : HRP conjugated at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 49 kDa
    Observed band size: 49 kDa

    Exposure time: 30 seconds

    A number of additional non-specific bands were also observed at 17, 33, 35 & 40kDa

    See Abreview

  • ab56023 staining mouse e18 skeleton tissues by immunohistochemistry (frozen sections). Sections were formaldehyde fixed prior to being blocked in 20% serum for 1 hour at RT. Sample was incubated with ab56023, diluted 1/1000, for 16 hours at 4°C. Alexa fluor® 680 donkey polyclonal, diluted 1/200, was used as the secondary antibody.

    See Abreview


This product has been referenced in:
  • Miao N  et al. Loss of Fam20c causes defects in the acinar and duct structure of salivary glands in mice. Int J Mol Med 43:2103-2117 (2019). Read more (PubMed: 30864688) »
  • Kim Y  et al. Evaluation of Mesenchymal Stem Cell Sheets Overexpressing BMP-7 in Canine Critical-Sized Bone Defects. Int J Mol Sci 19:N/A (2018). Read more (PubMed: 30018197) »
See all 19 Publications for this product

Customer reviews and Q&As

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1-3 of 3 Abreviews

Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (9% SDS-PAGE)
Rat Tissue lysate - whole (Rat liver)
Rat liver
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 10 2014

Western blot
Rat Tissue lysate - whole (Brain Lysate)
Loading amount
20 µg
Brain Lysate
Gel Running Conditions
Reduced Non-Denaturing (Native) (15% Gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Mar 24 2010

Immunohistochemistry (Frozen sections)
Mouse Tissue sections (e18 Skeleton)
e18 Skeleton
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Nov 04 2009

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