Western blot abreview for Anti-BMP7 antibody

Good
Abreviews
Application
Western blot
Sample
Rat Tissue lysate - whole (Brain Lysate)
Loading amount
20 µg
Specification
Brain Lysate
Gel Running Conditions
Reduced Non-Denaturing (Native) (15% Gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C

Other product details

Dilution
1/500
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST with 1% Milk

Secondary antibody

Name
Non-Abcam antibody was used: Dako Cytomation P0260
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/3000

Detection

Detection method
ECL+
Exposure
30 second(s)
Bands
Specific: 49 kDa Non-specific: 17, 33, 35, 40 kDa
Positive control
Rat brain whole tissue lysate (P8, P15, P21)

Additional data

Additional Notes
Lane 1: Rat Brain Whole Tissue (age P8) Lysate
Lane 2: Rat Brain Whole Tissue (age P15) Lysate
Lane 3: Rat Brain Whole Tissue (age P21) Lysate
Lane 4: Rat Brain Whole Tissue (age P21) Lysate
Abcam response
Many thanks for your WB data on ab56023 BMP7 antibody.

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Mar 24 2010

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