Western blot abreview for Anti-BMP7 antibody

Average
Abreviews
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (9% SDS-PAGE)
Sample
Rat Tissue lysate - whole (Rat liver)
Specification
Rat liver
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: in TBST
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: anti-Rabbit-HRP (H+L)
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000

Detection

Detection method
ECL Pico
Bands
Specific: 50 kDa
Exposure
3 minute(s) and 0 second(s)

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Submitted Nov 10 2014

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