Overview

  • Product name

  • Description

    Rabbit polyclonal to BMP7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Chicken, Human
  • Immunogen

    Synthetic peptide corresponding to Human BMP7 (C terminal).
    Database link: NM_001719

  • Positive control

    • Rat kidney tissue; Human kidney cell lysate

Applications

Our Abpromise guarantee covers the use of ab93636 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
  • Application notes
    IHC-P: 1/50 - 1/200.
    WB: 1/200 - 1/1000. Predicted molecular weight: 49 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis.
    • Tissue specificity

      Expressed in the kidney and bladder. Lower levels seen in the brain.
    • Sequence similarities

      Belongs to the TGF-beta family.
    • Developmental stage

      Expressed in the developing eye, brain and ear during embryogenesis.
    • Post-translational
      modifications

      Several N-termini starting at positions 293, 300, 315 and 316 have been identified by direct sequencing resulting in secretion of different mature forms (PubMed:17977014).
    • Cellular localization

      Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • BMP 7 antibody
      • BMP-7 antibody
      • Bmp7 antibody
      • BMP7_HUMAN antibody
      • Bone morphogenetic protein 7 antibody
      • Bone morphogenic protein 7 antibody
      • Eptotermin alfa antibody
      • OP 1 antibody
      • OP-1 antibody
      • OP1 antibody
      • Osteogenic protein 1 antibody
      see all

    Images

    • ab93636, at 1/100 dilution, staining secreted BMP7 in formalin-fixed, paraffin-embedded rat kidney tissue by Immunohistochemistry.
    • Anti-BMP7 antibody (ab93636) at 1/200 dilution + Human kidney cell lysate

      Predicted band size: 49 kDa

    References

    This product has been referenced in:

    • Zhang Y  et al. Effects of imposed defocus of opposite sign on temporal gene expression patterns of BMP4 and BMP7 in chick RPE. Exp Eye Res 109:98-106 (2013). Read more (PubMed: 23428741) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Question
    Answer

    Thank you for contacting us.

    The lab let me know that this antibody was raised against a peptide from near the C terminal residues of human BMP-7 protein (within residues 200-400).

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More
    Application
    Western blot
    Sample
    Chicken Tissue lysate - whole (retina, RPE, choroid)
    Loading amount
    20 µg
    Specification
    retina, RPE, choroid
    Gel Running Conditions
    Reduced Denaturing (NuPAGE 4-12% Bis-Tris Gel)
    Blocking step
    (agent) for 30 minute(s) · Concentration: 100% · Temperature: 25°C

    Yan Zhang

    Verified customer

    Submitted Feb 28 2012

    Answer

    Thank you for contacting Abcam. As you have requested, I have changed the status of your abreviews for ab6285 (abreview xxxx) and ab93939 (abreview xxxx) to an editing stage. This way you will be able to go back into those and change the information that we were speaking of. I have also created an Abreview discount code for ab93636 for use in WB on chiicken. Details of that are below. Please let me know if you have any questions. DISCOUNT CODE: xxxx Expiration date: xxxx I am very pleased to hear you would like to accept our offer and test ab93636 in WB on chicken. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview forWB on chicken and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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