Product nameAnti-BMP7 antibody [EPR23143-31]
See all BMP7 primary antibodies
DescriptionRabbit monoclonal [EPR23143-31] to BMP7
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P
Species reactivityReacts with: Human
Recombinant fragment within Human BMP7 aa 250 to the C-terminus. The exact sequence is proprietary.
Database link: P18075
- WB: MCF7 whole cell lysate. IP: MCF7 whole cell lysate.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab269586 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 49 kDa.|
FunctionInduces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis.
Tissue specificityExpressed in the kidney and bladder. Lower levels seen in the brain.
Sequence similaritiesBelongs to the TGF-beta family.
Developmental stageExpressed in the developing eye, brain and ear during embryogenesis.
modificationsSeveral N-termini starting at positions 293, 300, 315 and 316 have been identified by direct sequencing resulting in secretion of different mature forms (PubMed:17977014).
- Information by UniProt
- BMP 7 antibody
- BMP-7 antibody
- Bmp7 antibody
All lanes : Anti-BMP7 antibody [EPR23143-31] (ab269586) at 1/1000 dilution
Lane 1 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : MDA-MB-231 (human breast adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 49 kDa
Observed band size: 49 kDa
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:16419056, 12792780,11807825)
Negative control: MDA-MB-231 (PMID: 16419056)
This blot was developed using a higher sensitivity ECL substrate. Exposure time: 3 minutes
BMP7 was immunoprecipitated from 0.35 mg MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate 10µg with ab269586 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab269586 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
Lane 1: MCF7 whole cell lysate 10µg
Lane 2: ab269586 IP in MCF7 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab269586 in MCF7 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab269586 has not yet been referenced specifically in any publications.