Recombinant
RabMAb

Recombinant Anti-BMP7 antibody [EPR5897] (ab129156)

Overview

  • Product name

    Anti-BMP7 antibody [EPR5897]
    See all BMP7 primary antibodies
  • Description

    Rabbit monoclonal [EPR5897] to BMP7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human BMP7 aa 300-400. The exact sequence is proprietary.

  • Positive control

    • WB: Rat and mouse kidney lysate, Fetal kidney, Human prostate and HT-1376 lysates; recombinant Osteogenic Growth Peptide IP: MCF7 whole cell lysate
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab129156 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 49 kDa.
IP 1/150.

For unpurified use at 1/10 - 1/100.

  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Function

      Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis.
    • Tissue specificity

      Expressed in the kidney and bladder. Lower levels seen in the brain.
    • Sequence similarities

      Belongs to the TGF-beta family.
    • Developmental stage

      Expressed in the developing eye, brain and ear during embryogenesis.
    • Post-translational
      modifications

      Several N-termini starting at positions 293, 300, 315 and 316 have been identified by direct sequencing resulting in secretion of different mature forms (PubMed:17977014).
    • Cellular localization

      Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • BMP 7 antibody
      • BMP-7 antibody
      • Bmp7 antibody
      • BMP7_HUMAN antibody
      • Bone morphogenetic protein 7 antibody
      • Bone morphogenic protein 7 antibody
      • Eptotermin alfa antibody
      • OP 1 antibody
      • OP-1 antibody
      • OP1 antibody
      • Osteogenic protein 1 antibody
      see all

    Images

    • All lanes : Anti-BMP7 antibody [EPR5897] (ab129156) at 1/5000 dilution (purified)

      Lane 1 : Rat kidney lysate
      Lane 2 : Mouse kidney lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

      Predicted band size: 49 kDa
      Observed band size: 49 kDa



      Blocking/Diluting buffer 5% NFDM/TBST
    • Anti-BMP7 antibody [EPR5897] (ab129156) at 1/1000 dilution (purified) + MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 1/15 dilution

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 49 kDa
      Observed band size: 49 kDa



      Blocking/Diluting buffer 5% NFDM/TBST
    • Purified ab129156 at 1/150 immunoprecipitating BMP7 in MCF7 (Human breast adenocarcinoma cell line) whole cell lysate observed at 49 KDa (lanes 1 and 2).

      Lane 1 (input): MCF7 whole cell lysate 10μg

      Lane 2 (+): ab129156 + MCF7 whole cell lysate.

      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab129156 in MCF7 whole cell lysate

      For western blotting, ab129156 at 1/150 dilution and VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

      Blocking and Diluting buffer and concentration: 5% NFDM/TBST.

    • All lanes : Anti-BMP7 antibody [EPR5897] (ab129156) at 1/1000 dilution (unpurified)

      Lane 1 : Fetal kidney lysate
      Lane 2 : Human prostate lysate
      Lane 3 : HT-1376 lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 49 kDa

    • Anti-BMP7 antibody [EPR5897] (ab129156) at 1/1000 dilution (unpurified) + recombinant Human BMP7 (amino acids 316 - 431) at 0.01 µg

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 49 kDa



      The recombinant peptide should give a band of approximately 13.7 kDa.

    References

    This product has been referenced in:

    • Yao H  et al. BMP7 antagonizes proliferative vitreoretinopathy through retinal pigment epithelial fibrosis in vivo and in vitro. FASEB J N/A:fj201800858RR (2018). Read more (PubMed: 30383450) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (brain)
    Permeabilization
    Yes - 0.3% TritonX-100
    Specification
    brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Oct 27 2015

    Answer

    We unfortunately do not have data that would answer your customer's question about whether the antibody has more affinity for the BMP7 precursor or mature form. What is needed is a comparison of the recovery of each protein form (precursor and mature) in the IP product and in the flow-through fraction of proteins that are not precipitated.

    Read More

    Answer

    The BMP7 antibody ab129156 is raised against an amino acid sequence that is present in the precursor as well as the mature protein. For western blotting, the antibody should be able to detect both forms with equal affinity, if the samples are denatured and reduced. If experimental samples contain native, non-denatured protein, the antibody may have higher affinity for one or the other, depending on their native conformation, which will determine how available the amino acid sequence is for antibody recognition. We do not have data demonstrating this, though.

    We are not aware of any references that have used this antibody for IP of cartilage, or for any assay of BMP7 in cartilage or chondrocytes.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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