Recombinant
RabMAb

Recombinant Anti-BMP7 antibody [EPR5897] - BSA and Azide free (ab240045)

Overview

  • Product name

    Anti-BMP7 antibody [EPR5897] - BSA and Azide free
    See all BMP7 primary antibodies
  • Description

    Rabbit monoclonal [EPR5897] to BMP7 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human BMP7 aa 300-400. The exact sequence is proprietary.

  • General notes

    Ab240045 is the carrier-free version of ab129156. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab240045 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab240045 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 49 kDa.
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Function

      Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis.
    • Tissue specificity

      Expressed in the kidney and bladder. Lower levels seen in the brain.
    • Sequence similarities

      Belongs to the TGF-beta family.
    • Developmental stage

      Expressed in the developing eye, brain and ear during embryogenesis.
    • Post-translational
      modifications

      Several N-termini starting at positions 293, 300, 315 and 316 have been identified by direct sequencing resulting in secretion of different mature forms (PubMed:17977014).
    • Cellular localization

      Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • BMP 7 antibody
      • BMP-7 antibody
      • Bmp7 antibody
      • BMP7_HUMAN antibody
      • Bone morphogenetic protein 7 antibody
      • Bone morphogenic protein 7 antibody
      • Eptotermin alfa antibody
      • OP 1 antibody
      • OP-1 antibody
      • OP1 antibody
      • Osteogenic protein 1 antibody
      see all

    Images

    • Purified ab129156 at 1/150 immunoprecipitating BMP7 in MCF7 (Human breast adenocarcinoma cell line) whole cell lysate observed at 49 KDa (lanes 1 and 2).

      Lane 1 (input): MCF7 whole cell lysate 10μg

      Lane 2 (+): ab129156 + MCF7 whole cell lysate.

      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab129156 in MCF7 whole cell lysate

      For western blotting, ab129156 at 1/150 dilution and VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

      Blocking and Diluting buffer and concentration: 5% NFDM/TBST.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129156).

    References

    ab240045 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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