Product nameBMP7 overexpression 293T lysate (whole cell)
DescriptionBMP7 overexpression lysate
ab94187 is a 293T cell transfected lysate in which Human BMP7 has been transiently over-expressed using a pCMV-BMP7 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view the preparation notes.
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferConstituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine)
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BackgroundFunction: Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis. Tissue specificity: Expressed in the kidney and bladder. Lower levels seen in the brain. Similarity: Belongs to the TGF-beta family. Developmental stage: Expressed in the developing eye, brain and ear during embryogenesis. PTM: Several N-termini starting at positions 293, 300, 315 and 316 have been identified by direct sequencing resulting in secretion of different mature forms (PubMed:17977014).
Our Abpromise guarantee covers the use of ab94187 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94187 at 15 μg/lane on an SDS-PAGE gel
All lanes : Anti-BMP7 antibody (ab54904) at 1/500 dilution
Lane 1 :
BMP7 overexpression 293T lysate (whole cell) (ab94187)
Lane 2 : 293T non-transfected Lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94187 has not yet been referenced specifically in any publications.