Recombinant Anti-OP-2 antibody [EPR13357] (ab181253)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13357] to OP-2
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-OP-2 antibody [EPR13357]
See all OP-2 primary antibodies -
Description
Rabbit monoclonal [EPR13357] to OP-2 -
Host species
Rabbit -
Specificity
This antibody detects dominant ~35kDa extra band in some samples, e.g. WI-38 and SK-OV-3. -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa and A549 cell lysate; A549 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13357 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181253 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
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ICC/IF |
1/250.
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Notes |
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WB
1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa). |
ICC/IF
1/250. |
Target
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Function
Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis. -
Sequence similarities
Belongs to the TGF-beta family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 656 Human
- Omim: 602284 Human
- SwissProt: P34820 Human
- Unigene: 664022 Human
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Alternative names
- BMP-8 antibody
- BMP-8B antibody
- BMP8 antibody
see all
Images
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All lanes : Anti-OP-2 antibody [EPR13357] (ab181253) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A549 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 45 kDa
Observed band size: 45 kDa -
Immunofluorescence analysis of, paraformaldehyde-fixed, A549 cells labeling OP-2 with ab181253 at a 1/250 dilution. As secondary antibody goat anti rabbit IgG (Alexa Fluor® 488) was used at a 1/200 dilution. In blue DAPI staining.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab181253 has not yet been referenced specifically in any publications.