Product nameAnti-BMP9 antibody
See all BMP9 primary antibodies
DescriptionRabbit polyclonal to BMP9
Tested applicationsSuitable for: ELISA, IHC-P, ICC/IF, WB, IHC-Frmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse, Dog, Chimpanzee, Macaque monkey
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.2
Concentration information loading...
PurityProtein G purified
Purification notesAmmonium Sulphate fractionation.
Our Abpromise guarantee covers the use of ab35088 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||1/50 - 1/200.|
|IHC-P||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 20065063|
RelevanceBMP9 is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. They are regulators of cell growth and differentiation. BMP9 has a role in the maturation of BFCN (basal forebrain cholinergic neurons) also inducing and maintaining the ability of these cells to respond to acetylcholine; BFCN are important for the processes of learning, memory and attention. BMP9 is a potent inducer of hepcidin (a cationic peptide that has a antimicrobial properties) in hepatocytes and can regulate iron metabolism. The physiological receptor of BMP9 is thought to be activin receptor-like kinase 1, ALK1 (also called ACVRL1), an endothelial-specific type I receptor of the TGF-beta receptor family. BMP9 is one of the most potent BMPs to induce orthotopic bone formation in vivo. BMP3, a blocker of most BMPs seems not to affect BMP9.
- BMP 9 antibody
- Bone morphogenetic protein 9 antibody
- GDF 2 antibody
Immunofluorescent staining of BMP9 in rat primary hippocampus neurons with ab35088 (1/500). Cells were fixed with PFA and permeabilized with 0.3% Triton X in 0.1% PBS followed by a blocking step with 10% serum for 1 hour at 24oC. Incubation with ab35088 (1/500) was then performed during 2 hours at 24oC. The secondary antibody was an anti rabbit-Alexa Fluro 488. DAPI shows nuclear localization.
This product has been referenced in:
- Voorneveld PW et al. The BMP pathway either enhances or inhibits the Wnt pathway depending on the SMAD4 and p53 status in CRC. Br J Cancer 112:122-30 (2015). Read more (PubMed: 25393365) »
- Cunha SI et al. Endothelial ALK1 Is a Therapeutic Target to Block Metastatic Dissemination of Breast Cancer. Cancer Res 75:2445-56 (2015). Read more (PubMed: 26077471) »