Recombinant Anti-BMP9 antibody [EPR20009] (ab207318)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-BMP9 antibody [EPR20009]
See all BMP9 primary antibodies -
Description
Rabbit monoclonal [EPR20009] to BMP9 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human BMP9 aa 300 to the C-terminus. The exact sequence is proprietary.
Database link: Q9UK05 -
Positive control
- WB: GST-tagged Human BMP9 recombinant protein (aa338-429); Human liver lysate.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20009 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab207318 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa). |
Target
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Relevance
BMP9 is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. They are regulators of cell growth and differentiation. BMP9 has a role in the maturation of BFCN (basal forebrain cholinergic neurons) also inducing and maintaining the ability of these cells to respond to acetylcholine; BFCN are important for the processes of learning, memory and attention. BMP9 is a potent inducer of hepcidin (a cationic peptide that has a antimicrobial properties) in hepatocytes and can regulate iron metabolism. The physiological receptor of BMP9 is thought to be activin receptor-like kinase 1, ALK1 (also called ACVRL1), an endothelial-specific type I receptor of the TGF-beta receptor family. BMP9 is one of the most potent BMPs to induce orthotopic bone formation in vivo. BMP3, a blocker of most BMPs seems not to affect BMP9. -
Cellular localization
Secreted -
Database links
- Entrez Gene: 2658 Human
- Omim: 605120 Human
- SwissProt: Q9UK05 Human
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Alternative names
- BMP 9 antibody
- Bone morphogenetic protein 9 antibody
- GDF 2 antibody
see all
Images
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![Western blot - Anti-BMP9 antibody [EPR20009] (ab207318)](https://www.abcam.com/ps/products/207/ab207318/Images/ab207318-276127-anti-bmp9-antibody-epr20009-western-blot.jpg)
Western blot - Anti-BMP9 antibody [EPR20009] (ab207318)All lanes : Anti-BMP9 antibody [EPR20009] (ab207318) at 1/10000 dilution
Lane 1 : GST-tagged Human BMP9 recombinant protein (aa338-429)
Lane 2 : His-tagged Human BMP10 recombinant protein (aa334-424)
Lane 3 : His-tagged Human BMP13 recombinant protein (aa365-455)
Lysates/proteins at 0.02 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 47 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Exposure time: 4 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-BMP9 antibody [EPR20009] (ab207318)](https://www.abcam.com/ps/products/207/ab207318/Images/ab207318-276126-anti-bmp9-antibody-epr20009-western-blot.jpg)
Western blot - Anti-BMP9 antibody [EPR20009] (ab207318)All lanes : Anti-BMP9 antibody [EPR20009] (ab207318) at 1/1000 dilution
Lane 1 : Human liver lysate
Lane 2 : Human brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
BMP9 is mostly expressed in liver and has very low expression levels in brain and other tissues. PMID 21710321; PMID 23812757.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Tang S et al. LncRNA-OG Promotes the Osteogenic Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells Under the Regulation of hnRNPK. Stem Cells 37:270-283 (2019). Read more (PubMed: 30372559) »
