Recombinant Anti-BMP9 antibody [EPR20009] (ab207318)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20009] to BMP9
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-BMP9 antibody [EPR20009]
See all BMP9 primary antibodies -
Description
Rabbit monoclonal [EPR20009] to BMP9 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human BMP9 aa 300 to the C-terminus. The exact sequence is proprietary.
Database link: Q9UK05 -
Positive control
- WB: GST-tagged Human BMP9 recombinant protein (aa338-429); Human liver lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20009 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab207318 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | 1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa). |
Target
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Relevance
BMP9 is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. They are regulators of cell growth and differentiation. BMP9 has a role in the maturation of BFCN (basal forebrain cholinergic neurons) also inducing and maintaining the ability of these cells to respond to acetylcholine; BFCN are important for the processes of learning, memory and attention. BMP9 is a potent inducer of hepcidin (a cationic peptide that has a antimicrobial properties) in hepatocytes and can regulate iron metabolism. The physiological receptor of BMP9 is thought to be activin receptor-like kinase 1, ALK1 (also called ACVRL1), an endothelial-specific type I receptor of the TGF-beta receptor family. BMP9 is one of the most potent BMPs to induce orthotopic bone formation in vivo. BMP3, a blocker of most BMPs seems not to affect BMP9. -
Cellular localization
Secreted -
Database links
- Entrez Gene: 2658 Human
- Omim: 605120 Human
- SwissProt: Q9UK05 Human
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Alternative names
- BMP 9 antibody
- Bone morphogenetic protein 9 antibody
- GDF 2 antibody
see all
Images
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All lanes : Anti-BMP9 antibody [EPR20009] (ab207318) at 1/10000 dilution
Lane 1 : GST-tagged Human BMP9 recombinant protein (aa338-429)
Lane 2 : His-tagged Human BMP10 recombinant protein (aa334-424)
Lane 3 : His-tagged Human BMP13 recombinant protein (aa365-455)
Lysates/proteins at 0.02 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 47 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Exposure time: 4 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-BMP9 antibody [EPR20009] (ab207318) at 1/1000 dilution
Lane 1 : Human liver lysate
Lane 2 : Human brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
BMP9 is mostly expressed in liver and has very low expression levels in brain and other tissues. PMID 21710321; PMID 23812757.
Datasheets and documents
Certificate of Compliance
References (1)
ab207318 has been referenced in 1 publication.
- Tang S et al. LncRNA-OG Promotes the Osteogenic Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells Under the Regulation of hnRNPK. Stem Cells 37:270-283 (2019). PubMed: 30372559