Overview

  • Product name
  • Description
    Rabbit polyclonal to BMPR1A
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Goat, Cow, Cat, Pig, Chimpanzee, Macaque monkey, Gorilla
  • Immunogen

    Synthetic peptide corresponding to Human BMPR1A aa 1-100 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: P36894

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: MCF7; MDA MB 231; Caco2; LoVo; HeLa. This antibody gave a positive result in IHC in the following FFPE tissue: Normal human heart muscle.

Properties

Applications

Our Abpromise guarantee covers the use of ab174815 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 60 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for BMP2, BMP4, GDF5 and GDF6. Positively regulates chondrocyte differentiation through GDF5 interaction. Mediates induction of adipogenesis by GDF6.
  • Tissue specificity
    Highly expressed in skeletal muscle.
  • Involvement in disease
    Juvenile polyposis syndrome
    Polyposis syndrome, mixed hereditary 2
    A microdeletion of chromosome 10q23 involving BMPR1A and PTEN is a cause of chromosome 10q23 deletion syndrome, which shows overlapping features of the following three disorders: Bannayan-Zonana syndrome, Cowden disease and juvenile polyposis syndrome.
  • Sequence similarities
    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
    Contains 1 GS domain.
    Contains 1 protein kinase domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 10q23del antibody
    • Activin A receptor type II like kinase 3 antibody
    • Activin receptor like kinase 3 antibody
    • Activin receptor-like kinase 3 antibody
    • ACVRLK 3 antibody
    • ACVRLK3 antibody
    • ALK 3 antibody
    • ALK-3 antibody
    • ALK3 antibody
    • BMP type-1A receptor antibody
    • BMPR 1A antibody
    • Bmpr antibody
    • BMPR-1A antibody
    • Bmpr1a antibody
    • BMR1A_HUMAN antibody
    • Bone morphogenetic protein receptor type IA antibody
    • Bone morphogenetic protein receptor type IA precursor antibody
    • Bone morphogenetic protein receptor type-1A antibody
    • BR 1a antibody
    • BR1a antibody
    • CD 292 antibody
    • CD292 antibody
    • CD292 antigen antibody
    • EC 2.7.11.30 antibody
    • Serine threonine protein kinase receptor R5 antibody
    • Serine threonine protein kinase receptor R5 precursor antibody
    • Serine/threonine-protein kinase receptor R5 antibody
    • SKR 5 antibody
    • SKR5 antibody
    • zBMPR IA antibody
    • zBMPRIA antibody
    see all

Images

  • IHC image of TBMPR1A staining in Normal human heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab174815, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

     

  • All lanes : Anti-BMPR1A antibody (ab174815) at 1 µg/ml

    Lane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 2 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 3 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 4 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
    Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 1 µg/ml per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 60 kDa
    Observed band size: 58 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 65 kDa (possible non-specific binding)


    Exposure time: 2 minutes


    The band observed at 58 kDa could potentially be a cleaved form of BMPR1A due to the presence of a 23 amino acid signal peptide.

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab174815 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406

References

This product has been referenced in:
  • Song LL  et al. Expression characteristics of BMP2, BMPR-IA and Noggin in different stages of hair follicle in yak skin. Gen Comp Endocrinol 260:18-24 (2018). Read more (PubMed: 29174869) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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