Overview

  • Product name
  • Description
    Rabbit polyclonal to BMPR1A
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human BMPR1A aa 166-195 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • Positive control
    • WB: HeLa and 293 cell lysates. IHC-P: Human hepatocarcinoma tissue. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab38560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
IHC-Fr Use at an assay dependent concentration. PubMed: 25093411
ICC/IF Use a concentration of 5 µg/ml.
WB 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).

Target

  • Function
    On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for BMP2, BMP4, GDF5 and GDF6. Positively regulates chondrocyte differentiation through GDF5 interaction. Mediates induction of adipogenesis by GDF6.
  • Tissue specificity
    Highly expressed in skeletal muscle.
  • Involvement in disease
    Juvenile polyposis syndrome
    Polyposis syndrome, mixed hereditary 2
    A microdeletion of chromosome 10q23 involving BMPR1A and PTEN is a cause of chromosome 10q23 deletion syndrome, which shows overlapping features of the following three disorders: Bannayan-Zonana syndrome, Cowden disease and juvenile polyposis syndrome.
  • Sequence similarities
    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
    Contains 1 GS domain.
    Contains 1 protein kinase domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 10q23del antibody
    • Activin A receptor type II like kinase 3 antibody
    • Activin receptor like kinase 3 antibody
    • Activin receptor-like kinase 3 antibody
    • ACVRLK 3 antibody
    • ACVRLK3 antibody
    • ALK 3 antibody
    • ALK-3 antibody
    • ALK3 antibody
    • BMP type-1A receptor antibody
    • BMPR 1A antibody
    • Bmpr antibody
    • BMPR-1A antibody
    • Bmpr1a antibody
    • BMR1A_HUMAN antibody
    • Bone morphogenetic protein receptor type IA antibody
    • Bone morphogenetic protein receptor type IA precursor antibody
    • Bone morphogenetic protein receptor type-1A antibody
    • BR 1a antibody
    • BR1a antibody
    • CD 292 antibody
    • CD292 antibody
    • CD292 antigen antibody
    • EC 2.7.11.30 antibody
    • Serine threonine protein kinase receptor R5 antibody
    • Serine threonine protein kinase receptor R5 precursor antibody
    • Serine/threonine-protein kinase receptor R5 antibody
    • SKR 5 antibody
    • SKR5 antibody
    • zBMPR IA antibody
    • zBMPRIA antibody
    see all

Images

  • All lanes : Anti-BMPR1A antibody (ab38560) at 1000 cells

    Lane 1 : HEK293 cell line
    Lane 2 : Mouse NIH/3T3 cell line

    Lysates/proteins at 35 mg/ml per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG H&L(HRP) at 1/5000 dilution

    Predicted band size: 60 kDa

  • Anti-BMPR1A antibody (ab38560) at 1/1000 dilution + Hela cell line lysates at 35 µg

    Predicted band size: 60 kDa

  • Immunohistochemical (formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocarcinoma tissue labelling BMPR1A with ab38560. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at 38°C. Antigen retrieval was heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A peroxidase-conjugated goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.

  • ICC/IF image of ab38560 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38560, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Tengroth L  et al. Activation of Activin receptor-like kinases curbs mucosal inflammation and proliferation in chronic rhinosinusitis with nasal polyps. Sci Rep 8:1561 (2018). Read more (PubMed: 29367682) »
  • Mahadik K  et al. c-Abl-TWIST1 Epigenetically Dysregulate Inflammatory Responses during Mycobacterial Infection by Co-Regulating Bone Morphogenesis Protein and miR27a. Front Immunol 9:85 (2018). Read more (PubMed: 29449840) »
See all 9 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (HEK293 cells)
Specification
HEK293 cells
Treatment
FLAG, or FLAG-BMPR1A transfected
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Mar 11 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Rat aorta)
Loading amount
40 µg
Specification
Rat aorta
Gel Running Conditions
Reduced Denaturing (9% SDS-PAGE)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Chrispina Hong

Verified customer

Submitted Jan 18 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (intestine)
Specification
intestine
Fixative
Paraformaldehyde
Permeabilization
Yes - .01% triton x-100
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Apr 17 2012

Question
Answer



I am very pleased to hear you would like to accept our offer and test ab38560 in chicken. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forchicken and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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