Product nameAnti-BMPR1A antibody
See all BMPR1A primary antibodies
DescriptionRabbit polyclonal to BMPR1A
Tested applicationsSuitable for: IHC-P, IHC-Fr, ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human BMPR1A aa 166-195 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH).
- WB: HeLa and 293 cell lysates. IHC-P: Human hepatocarcinoma tissue. ICC/IF: HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Concentration information loading...
PurityAmmonium Sulphate Precipitation
Purification notesThis antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Our Abpromise guarantee covers the use of ab38560 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 25093411|
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).|
FunctionOn ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for BMP2, BMP4, GDF5 and GDF6. Positively regulates chondrocyte differentiation through GDF5 interaction. Mediates induction of adipogenesis by GDF6.
Tissue specificityHighly expressed in skeletal muscle.
Involvement in diseaseJuvenile polyposis syndrome
Polyposis syndrome, mixed hereditary 2
A microdeletion of chromosome 10q23 involving BMPR1A and PTEN is a cause of chromosome 10q23 deletion syndrome, which shows overlapping features of the following three disorders: Bannayan-Zonana syndrome, Cowden disease and juvenile polyposis syndrome.
Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
Contains 1 GS domain.
Contains 1 protein kinase domain.
- Information by UniProt
- 10q23del antibody
- Activin A receptor type II like kinase 3 antibody
- Activin receptor like kinase 3 antibody
All lanes : Anti-BMPR1A antibody (ab38560) at 1000 cells
Lane 1 : HEK293 cell line
Lane 2 : Mouse NIH/3T3 cell line
Lysates/proteins at 35 mg/ml per lane.
All lanes : Goat anti-rabbit IgG H&L(HRP) at 1/5000 dilution
Predicted band size: 60 kDa
Anti-BMPR1A antibody (ab38560) at 1/1000 dilution + Hela cell line lysates at 35 µg
Predicted band size: 60 kDa
Immunohistochemical (formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocarcinoma tissue labelling BMPR1A with ab38560. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at 38°C. Antigen retrieval was heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A peroxidase-conjugated goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.
ICC/IF image of ab38560 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38560, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- Tengroth L et al. Activation of Activin receptor-like kinases curbs mucosal inflammation and proliferation in chronic rhinosinusitis with nasal polyps. Sci Rep 8:1561 (2018). Read more (PubMed: 29367682) »
- Mahadik K et al. c-Abl-TWIST1 Epigenetically Dysregulate Inflammatory Responses during Mycobacterial Infection by Co-Regulating Bone Morphogenesis Protein and miR27a. Front Immunol 9:85 (2018). Read more (PubMed: 29449840) »