Key features and details
- Rabbit polyclonal to BMPR1B
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-BMPR1B antibody
See all BMPR1B primary antibodies
DescriptionRabbit polyclonal to BMPR1B
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Chicken
Recombinant full length protein corresponding to Human BMPR1B aa 1-502.
MLLRSAGKLNVGTKKEDGESTAPTPRPKVLRCKCHHHCPEDSVNNICSTD GYCFTMIEEDDSGLPVVTSGCLGLEGSDFQCRDTPIPHQRRSIECCTERN ECNKDLHPTLPPLKNRDFVDGPIHHRALLISVTVCSLLLVLIILFCYFRY KRQETRPRYSIGLEQDETYIPPGESLRDLIEQSQSSGSGSGLPLLVQRTI AKQIQMVKQIGKGRYGEVWMGKWRGEKVAVKVFFTTEEASWFRETEIYQT VLMRHENILGFIAADIKGTGSWTQLYLITDYHENGSLYDYLKSTTLDAKS MLKLAYSSVSGLCHLHTEIFSTQGKPAIAHRDLKSKNILVKKNGTCCIAD LGLAVKFISDTNEVDIPPNTRVGTKRYMPPEVLDESLNRNHFQSYIMADM YSFGLILWEVARRCVSGGIVEEYQLPYHDLVPSDPSYEDMREIVCIKKLR PSFPNRWSSDECLRQMGKLMTECWAHNPASRLTALRVKKTLAKMSESQDI KL
Database link: O00238
- WB: U-251MG cell lysate and Mouse kidney tissue lysate. IHC: human gastric cancer and human tonsil. Rat ovary and mouse liver. ICC/IF: L929 cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab175385 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/200.|
|IHC-P||1/50 - 1/200.|
|WB||1/500 - 1/2000. Predicted molecular weight: 57 kDa.|
FunctionOn ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for BMP7/OP-1 and GDF5.
Involvement in diseaseDefects in BMPR1B are the cause of acromesomelic chondrodysplasia with genital anomalies (AMDGA) [MIM:609441]. Acromesomelic chondrodysplasias are rare hereditary skeletal disorders characterized by short stature, very short limbs, and hand/foot malformations. The severity of limb abnormalities increases from proximal to distal with profoundly affected hands and feet showing brachydactyly and/or rudimentary fingers (knob-like fingers).
Defects in BMPR1B are a cause of brachydactyly type A2 (BDA2) [MIM:112600]. Brachydactylies (BDs) are a group of inherited malformations characterized by shortening of the digits due to abnormal development of the phalanges and/or the metacarpals. They have been classified on an anatomic and genetic basis into five groups, A to E, including three subgroups (A1 to A3) that usually manifest as autosomal dominant traits. BDA2 was described first in a large Norwegian kindred. BDA2 is caused by mutations in BMPR1B gene and studies demonstrate that these mutations function as dominant negatives in vitro and in vivo.
Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
Contains 1 GS domain.
Contains 1 protein kinase domain.
- Information by UniProt
- Activin receptor like kinase 6 antibody
- Acvrlk6 antibody
- ALK 6 antibody
All lanes : Anti-BMPR1B antibody (ab175385) at 1/1000 dilution
Lane 1 : U-251MG cell lysate
Lane 2 : Mouse kidney tissue lysate
Lysates/proteins at 25 µg per lane.
All lanes : HRP Goat AntiRabbit IgG (H+L) at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 57 kDa
Formalin-fixed, paraffin-embedded human gastric cancer tissue stained for BMPR1B using ab175385 at 1/200 dilution in immunohistochemical analysis.
Immunofluorescence staining of L929 cells stained for BMPR1B with ab175385 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).
Formalin-fixed, paraffin-embedded human tonsil tissue stained for BMPR1B using ab175385 at 1/200 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded mouse liver tissue stained for BMPR1B using ab175385 at 1/200 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded rat ovary tissue stained for BMPR1B using ab175385 at 1/200 dilution in immunohistochemical analysis.
ab175385 has been referenced in 2 publications.
- Wood CL et al. A comparison of the bone and growth phenotype of mdx, mdx:Cmah-/- and mdx:Utrn+/- murine models with the C57BL/10 wild-type mouse. Dis Model Mech 13:N/A (2020). PubMed: 31754018
- O'Keeffe GW et al. Region-specific role of growth differentiation factor-5 in the establishment of sympathetic innervation. Neural Dev 11:4 (2016). ICC . PubMed: 26878848