Overview

  • Product name

    Anti-BMPR2 antibody [1F12]
    See all BMPR2 primary antibodies
  • Description

    Mouse monoclonal [1F12] to BMPR2
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, African green monkey
  • Immunogen

    Purified recombinant fragment of Human BMPR2 expressed in E. Coli.

  • Positive control

    • Breast, stomach and kidney cancer tissue; tonsil tissues. A431, NIH/3T3, Cos7 and PC-12 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab130206 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 115 kDa.
IHC-P 1/200 - 1/1000.
Flow Cyt Use 0.1-1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function

    On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Binds to BMP-7, BMP-2 and, less efficiently, BMP-4. Binding is weak but enhanced by the presence of type I receptors for BMPs.
  • Tissue specificity

    Highly expressed in heart and liver.
  • Involvement in disease

    Defects in BMPR2 are the cause of primary pulmonary hypertension (PPH1) [MIM:178600]. PPH1 is a rare autosomal dominant disorder characterized by plexiform lesions of proliferating endothelial cells in pulmonary arterioles. The lesions lead to elevated pulmonary arterial pression, right ventricular failure, and death. The disease can occur from infancy throughout life and it has a mean age at onset of 36 years. Penetrance is reduced. Although familial PPH1 is rare, cases secondary to known etiologies are more common and include those associated with the appetite-suppressant drugs.
    Defects in BMPR2 are a cause of pulmonary venoocclusive disease (PVOD) [MIM:265450]. PVOD is a rare form of pulmonary hypertension in which the vascular changes originate in the small pulmonary veins and venules. The pathogenesis is unknown and any link with PPH1 has been speculative. The finding of PVOD associated with a BMPR2 mutation reveals a possible pathogenetic connection with PPH1.
  • Sequence similarities

    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
    Contains 1 protein kinase domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • BMP type II receptor antibody
    • BMP type-2 receptor antibody
    • BMPR 2 antibody
    • BMPR 3 antibody
    • BMPR II antibody
    • BMPR-2 antibody
    • BMPR-II antibody
    • Bmpr2 antibody
    • BMPR2_HUMAN antibody
    • BMPR3 antibody
    • BMPRII antibody
    • BMR 2 antibody
    • BMR2 antibody
    • Bone morphogenetic protein receptor type 2 antibody
    • Bone morphogenetic protein receptor type II antibody
    • Bone morphogenetic protein receptor type-2 antibody
    • Bone morphogenic protein receptor type II serine threonine kinase antibody
    • BRK 3 antibody
    • BRK3 antibody
    • PPH 1 antibody
    • PPH1 antibody
    • Serine threonine kinase type II activin receptor like kinase antibody
    • T ALK antibody
    • TALK antibody
    • Type II activin receptor like kinase antibody
    see all

Images

  • All lanes : Anti-BMPR2 antibody [1F12] (ab130206) at 1/500 dilution

    Lane 1 : A431 cell lysate
    Lane 2 : NIH/3T3 cell lysate
    Lane 3 : Cos7 cell lysate
    Lane 4 : PC12 cell lysate
    Lane 5 : Cell lysate

    Predicted band size: 115 kDa

  • Overlay histogram showing HepG2 cells stained with ab130206 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab130206, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • ab130206 at 1/200 staining BMPR2 in paraffin-embedded tonsil tissue by immunohistochemistry with DAB staining.

References

This product has been referenced in:

  • Li Y  et al. Overexpression of Opa interacting protein 5 increases the progression of liver cancer via BMPR2/JUN/CHEK1/RAC1 dysregulation. Oncol Rep 41:2075-2088 (2019). Read more (PubMed: 30816485) »
  • Wang C  et al. Peroxisome Proliferator-Activated Receptor-? Knockdown Impairs Bone Morphogenetic Protein-2-Induced Critical-Size Bone Defect Repair. Am J Pathol N/A:N/A (2018). Read more (PubMed: 30593824) »
See all 4 Publications for this product

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