Overview

  • Product name

  • Description

    Rabbit polyclonal to BMPR2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinate fragment, corresponding to amino acids 295 - 552 of Human BMPR2 (BC052985).

  • Positive control

    • Raji cell lysate IF/ICC: HeLa cell line

Properties

Applications

Our Abpromise guarantee covers the use of ab106266 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/1000. Predicted molecular weight: 115 kDa.
IHC-P Use at an assay dependent concentration.

Target

  • Function

    On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Binds to BMP-7, BMP-2 and, less efficiently, BMP-4. Binding is weak but enhanced by the presence of type I receptors for BMPs.
  • Tissue specificity

    Highly expressed in heart and liver.
  • Involvement in disease

    Defects in BMPR2 are the cause of primary pulmonary hypertension (PPH1) [MIM:178600]. PPH1 is a rare autosomal dominant disorder characterized by plexiform lesions of proliferating endothelial cells in pulmonary arterioles. The lesions lead to elevated pulmonary arterial pression, right ventricular failure, and death. The disease can occur from infancy throughout life and it has a mean age at onset of 36 years. Penetrance is reduced. Although familial PPH1 is rare, cases secondary to known etiologies are more common and include those associated with the appetite-suppressant drugs.
    Defects in BMPR2 are a cause of pulmonary venoocclusive disease (PVOD) [MIM:265450]. PVOD is a rare form of pulmonary hypertension in which the vascular changes originate in the small pulmonary veins and venules. The pathogenesis is unknown and any link with PPH1 has been speculative. The finding of PVOD associated with a BMPR2 mutation reveals a possible pathogenetic connection with PPH1.
  • Sequence similarities

    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
    Contains 1 protein kinase domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • BMP type II receptor antibody
    • BMP type-2 receptor antibody
    • BMPR 2 antibody
    • BMPR 3 antibody
    • BMPR II antibody
    • BMPR-2 antibody
    • BMPR-II antibody
    • Bmpr2 antibody
    • BMPR2_HUMAN antibody
    • BMPR3 antibody
    • BMPRII antibody
    • BMR 2 antibody
    • BMR2 antibody
    • Bone morphogenetic protein receptor type 2 antibody
    • Bone morphogenetic protein receptor type II antibody
    • Bone morphogenetic protein receptor type-2 antibody
    • Bone morphogenic protein receptor type II serine threonine kinase antibody
    • BRK 3 antibody
    • BRK3 antibody
    • PPH 1 antibody
    • PPH1 antibody
    • Serine threonine kinase type II activin receptor like kinase antibody
    • T ALK antibody
    • TALK antibody
    • Type II activin receptor like kinase antibody
    see all

Images

  • ICC/IF image of ab106266 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab106266, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-BMPR2 antibody (ab106266) at 1/500 dilution + Raji cell lysate

    Predicted band size: 115 kDa

References

This product has been referenced in:

  • Xu X  et al. Endocardial fibroelastosis is caused by aberrant endothelial to mesenchymal transition. Circ Res 116:857-66 (2015). Read more (PubMed: 25587097) »
  • Gao X  et al. Bone morphogenetic protein signaling protects against cerulein-induced pancreatic fibrosis. PLoS One 9:e89114 (2014). Mouse . Read more (PubMed: 24586530) »
See all 3 Publications for this product

Customer reviews and Q&As

Answer

Thank you for your enquiry which has been forwarded to the scientific support team. I am sorry to confirm that because we carry over 70,000 products, it is not feasible for us to keep small sample sizes of our products to send to cusotmers. We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase. With regards to ab6285 Anti-BMP2 antibody [65529.111], I have reviewed the alingment of the immunogen for this antibody to the Zebrafish protein. There is 63% alignment to zebrafish. This alignment is quite low and I would therefore not recommend that this antibody is likely to detect the Zebrafish BMP2. The other antibodies we have to BMP2 are raised against full length human protein or shorter peptides from the human sequence, and they all have the same alignment or less. I am sorry there are no BMP2 antibodies to suggest that may be suitable on this occasion. With regards to BMPR2, the immunogen of ab78422 BMPR2 has only 58% alignment to zebrafish. Again, I am sorry this means it is not likely to crossreact with Zebrafish. However, we do have the following BMPR2 antibody which has 88% alignment to the zebrafish protein. I can recommend to review the online datasheet for further details. BMPR2 antibody (ab106266) https://www.abcam.com/index.html?datasheet=106266 https://www.abcam.com/index.html?datasheet=106266. If you would like to test this antibody in Zebrafish, you may be eligible for our testing discount program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information. Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates. To find out more about our Abreview system, please see the following link: https://www.abcam.com/abreviews I hope this information is helpful. Please do not hesitate to contact me again with any other questions.

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