Anti-BMPR2 antibody [MM0060-9A10] (ab78422)
Key features and details
- Mouse monoclonal [MM0060-9A10] to BMPR2
- Suitable for: Flow Cyt, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
-
Product name
Anti-BMPR2 antibody [MM0060-9A10]
See all BMPR2 primary antibodies -
Description
Mouse monoclonal [MM0060-9A10] to BMPR2 -
Host species
Mouse -
Specificity
ab78422 detects BMPR2. No cross reactivity was found to BMPR1A and BMPR1B. -
Tested applications
Suitable for: Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant human BMPR2 extracellular domain
-
Positive control
- IHC-P: Human placenta tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: PBS -
Concentration information loading...
-
Purity
Protein G purified -
Purification notes
The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and lyophilized from a 0.2 µm filtered solution in phosphate buffered saline (PBS). -
Clonality
Monoclonal -
Clone number
MM0060-9A10 -
Isotype
IgG1 -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab78422 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use 1-2µg for 106 cells.
(methanol or paraformaldehyde fixed cells)
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
|
IHC-P |
1/50 - 1/200. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. We recommend to use Proteinase K for the enzymatic antigen retrieval.
|
Notes |
---|
Flow Cyt
Use 1-2µg for 106 cells. (methanol or paraformaldehyde fixed cells)
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
IHC-P
1/50 - 1/200. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. We recommend to use Proteinase K for the enzymatic antigen retrieval. |
Target
-
Function
On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Binds to BMP-7, BMP-2 and, less efficiently, BMP-4. Binding is weak but enhanced by the presence of type I receptors for BMPs. -
Tissue specificity
Highly expressed in heart and liver. -
Involvement in disease
Defects in BMPR2 are the cause of primary pulmonary hypertension (PPH1) [MIM:178600]. PPH1 is a rare autosomal dominant disorder characterized by plexiform lesions of proliferating endothelial cells in pulmonary arterioles. The lesions lead to elevated pulmonary arterial pression, right ventricular failure, and death. The disease can occur from infancy throughout life and it has a mean age at onset of 36 years. Penetrance is reduced. Although familial PPH1 is rare, cases secondary to known etiologies are more common and include those associated with the appetite-suppressant drugs.
Defects in BMPR2 are a cause of pulmonary venoocclusive disease (PVOD) [MIM:265450]. PVOD is a rare form of pulmonary hypertension in which the vascular changes originate in the small pulmonary veins and venules. The pathogenesis is unknown and any link with PPH1 has been speculative. The finding of PVOD associated with a BMPR2 mutation reveals a possible pathogenetic connection with PPH1. -
Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
Contains 1 protein kinase domain. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 659 Human
- Omim: 600799 Human
- SwissProt: Q13873 Human
- Unigene: 471119 Human
-
Alternative names
- BMP type II receptor antibody
- BMP type-2 receptor antibody
- BMPR 2 antibody
see all
Images
-
ab78422 at 1/200 dilution, staining BMPR2 in human placental tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).
-
Overlay histogram showing HepG2 cells stained with ab78422 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab78422, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.
Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Protocols
Datasheets and documents
-
Datasheet download
References (12)
ab78422 has been referenced in 12 publications.
- Shi XF et al. Lysine (K)-specific demethylase 5C regulates the incidence of severe preeclampsia by adjusting the expression of bone morphogenetic protein-7. Bioengineered 13:8538-8547 (2022). PubMed: 35331081
- Wu CK et al. BMP2 promotes lung adenocarcinoma metastasis through BMP receptor 2-mediated SMAD1/5 activation. Sci Rep 12:16310 (2022). PubMed: 36175474
- Ren T et al. Osteosarcoma cell intrinsic PD-L2 signals promote invasion and metastasis via the RhoA-ROCK-LIMK2 and autophagy pathways. Cell Death Dis 10:261 (2019). PubMed: 30886151
- Wang S et al. BMPR2 and HIF1-aoverexpression in resected osteosarcoma correlates with distant metastasis and patient survival. Chin J Cancer Res 29:447-454 (2017). PubMed: 29142464
- Wang S et al. BMPR2 promotes invasion and metastasis via the RhoA-ROCK-LIMK2 pathway in human osteosarcoma cells. Oncotarget 8:58625-58641 (2017). PubMed: 28938584