Key features and details
- Rabbit polyclonal to BMX (phospho Y566)
- Suitable for: IHC-P, ELISA, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-BMX (phospho Y566) antibody
See all BMX primary antibodies
DescriptionRabbit polyclonal to BMX (phospho Y566)
SpecificityThis antibody detects endogenous levels of ETK only when phosphorylated at tyrosine 566.
Tested applicationsSuitable for: IHC-P, ELISA, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic phosphopeptide derived from human BMX around the phosphorylation site of tyrosine 566 (DQYPVS).
- Human thyroid gland tissue
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg+2 and Ca+2
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAffinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab59409 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|WB||1/3000. Detects a band of approximately 74 kDa (predicted molecular weight: 78 kDa).|
FunctionActivity is required for interleukin 6 (IL-6) induced differentiation. May play a role in the growth and differentiation of hematopoietic cells. May be involved in signal transduction in endocardial and arterial endothelial cells.
Tissue specificityPreferentially expressed in epithelial and endothelial cells.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
Contains 1 Btk-type zinc finger.
Contains 1 PH domain.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
DomainSH2 domain mediates interaction with RUFY1.
- Information by UniProt
- Bmx antibody
- BMX non receptor tyrosine kinase antibody
- BMX_HUMAN antibody
Immunohistochemical analysis of paraffin embedded human thyroid gland tissue using ab59409 at a 1/50 dilution, in the presence (right) and absence (left) of immunizing phosphopeptide.
Anti-BMX (phospho Y566) antibody (ab59409) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 78 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?
All lanes : Anti-BMX (phospho Y566) antibody (ab59409)
Lane 1 : Extracts from HeLa cells treated with Serum 20% 15'
Lane 2 : Extracts from HeLa cells treated with Serum 20% 15' and with the synthesized peptide.
Predicted band size: 78 kDa
ab59409 has been referenced in 2 publications.
- Holopainen T et al. Endothelial Bmx tyrosine kinase activity is essential for myocardial hypertrophy and remodeling. Proc Natl Acad Sci U S A 112:13063-8 (2015). PubMed: 26430242
- Guryanova OA et al. Nonreceptor Tyrosine Kinase BMX Maintains Self-Renewal and Tumorigenic Potential of Glioblastoma Stem Cells by Activating STAT3. Cancer Cell 19:498-511 (2011). WB, ICC/IF ; Mouse . PubMed: 21481791