Key features and details
- Rabbit polyclonal to BNIP3 - Carboxyterminal end
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-BNIP3 antibody - Carboxyterminal end
See all BNIP3 primary antibodies
DescriptionRabbit polyclonal to BNIP3 - Carboxyterminal end
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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FormLyophilized:0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab65874 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 30, 60 kDa (predicted molecular weight: 22 kDa).|
|ICC/IF||Use a concentration of 1 µg/ml.|
FunctionApoptosis-inducing protein that, which can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2.
Sequence similaritiesBelongs to the NIP3 family.
Cellular localizationMitochondrion. Mitochondrion membrane. Coexpression with the EIB 19-kDa protein results in a shift in NIP3 localization pattern to the nuclear envelope. Colocalizes with ACAA2 in the mitochondria.
- Information by UniProt
- BCL2 Adenovirus E1B 19kDa Interacting Protein 3 antibody
- BCL2/adenovirus E1B 19 kDa protein interacting protein 3 antibody
- BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 antibody
Anti-BNIP3 antibody - Carboxyterminal end (ab65874) at 2 µg/ml + rat thymus tissue lysate at 30 µg
Predicted band size: 22 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Additional bands at: 60 kDa (possible dimer)
ICC/IF image of ab65874 stained Mcf7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65874, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab65874 has been referenced in 4 publications.
- Salem AF et al. Cigarette smoke metabolically promotes cancer, via autophagy and premature aging in the host stromal microenvironment. Cell Cycle 12:818-25 (2013). PubMed: 23388463
- Fedorova LV et al. Peroxisome proliferator-activated receptor d agonist, HPP593, prevents renal necrosis under chronic ischemia. PLoS One 8:e64436 (2013). IHC-P ; Rat . PubMed: 23691217
- Fedorova LV et al. Mitochondrial impairment in the five-sixth nephrectomy model of chronic renal failure: proteomic approach. BMC Nephrol 14:209 (2013). WB ; Rat . PubMed: 24090408
- Kalas W et al. H-ras Up-regulates Expression of BNIP3. Anticancer Res 31:2869-75 (2011). WB ; Human . PubMed: 21868531