Overview

  • Product name

  • Description

    Rabbit polyclonal to BNIP3L/NIX
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Cow, Monkey
  • Immunogen

    Synthetic peptide corresponding to Human BNIP3L/NIX aa 95-189.
    Database link: O60238

  • Positive control

    • HeLa cells; Molt-4 whole cell lysate; Raji cells; mouse liver whole cell lysate; Human Colon cancer
  • General notes

     This product was previously labelled as BNIP3L

     

Properties

Applications

Our Abpromise guarantee covers the use of ab155010 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 24 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Induces apoptosis. Interacts with viral and cellular anti-apoptosis proteins. Can overcome the suppressors BCL-2 and BCL-XL, although high levels of BCL-XL expression will inhibit apoptosis. May function as a tumor suppressor. Inhibits apoptosis induced by BNIP3.
  • Sequence similarities

    Belongs to the NIP3 family.
  • Post-translational
    modifications

    Undergoes progressive proteolysis to an 11 kDa C-terminal fragment, which is blocked by the proteasome inhibitor lactacystin.
  • Cellular localization

    Nucleus envelope. Endoplasmic reticulum. Mitochondrion. Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Adenovirus E1B19k binding protein B5 antibody
    • Adenovirus E1B19K-binding protein B5 antibody
    • BCL2/adenovirus E1B 19 kd protein interacting protein 3a antibody
    • BCL2/adenovirus E1B 19 kDa protein interacting protein 3A antibody
    • BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like antibody
    • BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A antibody
    • BCL2/adenovirus E1B 19kDa interacting protein 3 like antibody
    • BNI3L_HUMAN antibody
    • BNIP3a antibody
    • BNIP3H antibody
    • BNIP3L antibody
    • BNIP3L protein antibody
    • NIP3 like protein X antibody
    • NIP3-like protein X antibody
    • NIP3L antibody
    • NIX antibody
    see all

Images

  • Anti-BNIP3L/NIX antibody (ab155010) at 1/1000 dilution + Molt4 whole cell lysate at 30 µg

    Predicted band size: 24 kDa



    12% SDS PAGE
  • Anti-BNIP3L/NIX antibody (ab155010) at 1/500 dilution + mouse liver whole cell lysate at 50 µg

    Predicted band size: 24 kDa



    12% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human colon cancer labeling BNIP3L/NIX with ab155010 antibody at 1/500 dilution.

  • Immunofluorescent analysis of methanol-fixed HeLa cells labeling BNIP3L/NIX with ab155010 antibody at 1/200 dilution. Bottom figure: staining merged with DNA probe (Hoechst 33342).

References

ab155010 has not yet been referenced specifically in any publications.

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