Product nameAnti-BNIP3L/NIX antibody
See all BNIP3L/NIX primary antibodies
DescriptionRabbit polyclonal to BNIP3L/NIX
Tested applicationsSuitable for: IPmore details
Unsuitable for: WB
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow
Synthetic peptide within Human BNIP3L/NIX aa 75-125. The exact sequence is proprietary. NP_004322.1
Database link: O60238
- IP: Jurkat whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264311 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
FunctionInduces apoptosis. Interacts with viral and cellular anti-apoptosis proteins. Can overcome the suppressors BCL-2 and BCL-XL, although high levels of BCL-XL expression will inhibit apoptosis. May function as a tumor suppressor. Inhibits apoptosis induced by BNIP3.
Sequence similaritiesBelongs to the NIP3 family.
modificationsUndergoes progressive proteolysis to an 11 kDa C-terminal fragment, which is blocked by the proteasome inhibitor lactacystin.
Cellular localizationNucleus envelope. Endoplasmic reticulum. Mitochondrion. Membrane.
- Information by UniProt
- Adenovirus E1B19k binding protein B5 antibody
- Adenovirus E1B19K-binding protein B5 antibody
- BCL2/adenovirus E1B 19 kd protein interacting protein 3a antibody
BNIP3L/NIX was immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate with ab264311 at 3 µg per reaction. Western blot was performed from the immunoprecipitate using ab264311 at 1 µg/ml.
Lane 1: ab264311 IP in Jurkat whole cell lysate.
Lane 2: Control IgG.
Exposure time: 3 mins.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264311 has not yet been referenced specifically in any publications.