Recombinant
RabMAb

Recombinant Anti-BNIP3L/NIX antibody [EPR4033] (ab109414)

Overview

  • Product name

    Anti-BNIP3L/NIX antibody [EPR4033]
    See all BNIP3L/NIX primary antibodies
  • Description

    Rabbit monoclonal [EPR4033] to BNIP3L/NIX
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human BNIP3L/NIX aa 200-300 (C terminal). The exact sequence is proprietary.

  • Positive control

    • Raji and K562 cell lysates; Human breast carcinoma tissue
  • General notes

     

     

     This product was previously labelled as BNIP3L

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109414 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 24 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

antigen retrieval is recommended.

  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function

      Induces apoptosis. Interacts with viral and cellular anti-apoptosis proteins. Can overcome the suppressors BCL-2 and BCL-XL, although high levels of BCL-XL expression will inhibit apoptosis. May function as a tumor suppressor. Inhibits apoptosis induced by BNIP3.
    • Sequence similarities

      Belongs to the NIP3 family.
    • Post-translational
      modifications

      Undergoes progressive proteolysis to an 11 kDa C-terminal fragment, which is blocked by the proteasome inhibitor lactacystin.
    • Cellular localization

      Nucleus envelope. Endoplasmic reticulum. Mitochondrion. Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Adenovirus E1B19k binding protein B5 antibody
      • Adenovirus E1B19K-binding protein B5 antibody
      • BCL2/adenovirus E1B 19 kd protein interacting protein 3a antibody
      • BCL2/adenovirus E1B 19 kDa protein interacting protein 3A antibody
      • BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like antibody
      • BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A antibody
      • BCL2/adenovirus E1B 19kDa interacting protein 3 like antibody
      • BNI3L_HUMAN antibody
      • BNIP3a antibody
      • BNIP3H antibody
      • BNIP3L antibody
      • BNIP3L protein antibody
      • NIP3 like protein X antibody
      • NIP3-like protein X antibody
      • NIP3L antibody
      • NIX antibody
      see all

    Images

    • All lanes : Anti-BNIP3L/NIX antibody [EPR4033] (ab109414) at 1/1000 dilution

      Lane 1 : Raji cell lysate
      Lane 2 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 24 kDa
      Observed band size: 35 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 1/100 ab109414

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    • Prieto-Domínguez N  et al. Melatonin enhances sorafenib actions in human hepatocarcinoma cells by inhibiting mTORC1/p70S6K/HIF-1a and hypoxia-mediated mitophagy. Oncotarget 8:91402-91414 (2017). Read more (PubMed: 29207653) »
    • Zhang W & Zhang J Dexmedetomidine preconditioning protects against lung injury induced by ischemia-reperfusion through inhibition of autophagy. Exp Ther Med 14:973-980 (2017). Read more (PubMed: 28810549) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Lung)
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Loading amount
    15 µg
    Treatment
    10^7 CFU S. Aureus peritoneal clot
    Specification
    Lung
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

    Mr. Alan Chang

    Verified customer

    Submitted Mar 20 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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