Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4033] to BNIP3L/NIX - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
Product nameAnti-BNIP3L/NIX antibody [EPR4033] - BSA and Azide free
See all BNIP3L/NIX primary antibodies
DescriptionRabbit monoclonal [EPR4033] to BNIP3L/NIX - BSA and Azide free
Tested applicationsSuitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt,ICC or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human BNIP3L/NIX aa 200-300 (C terminal). The exact sequence is proprietary.
ab239976 is the carrier-free version of ab109414 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab239976 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as BNIP3L
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab239976 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Antigen retrieval is recommended.
|WB||Use at an assay dependent concentration. Detects a band of approximately 35 kDa (predicted molecular weight: 24 kDa).|
FunctionInduces apoptosis. Interacts with viral and cellular anti-apoptosis proteins. Can overcome the suppressors BCL-2 and BCL-XL, although high levels of BCL-XL expression will inhibit apoptosis. May function as a tumor suppressor. Inhibits apoptosis induced by BNIP3.
Sequence similaritiesBelongs to the NIP3 family.
modificationsUndergoes progressive proteolysis to an 11 kDa C-terminal fragment, which is blocked by the proteasome inhibitor lactacystin.
Cellular localizationNucleus envelope. Endoplasmic reticulum. Mitochondrion. Membrane.
- Information by UniProt
- Adenovirus E1B19k binding protein B5 antibody
- Adenovirus E1B19K-binding protein B5 antibody
- BCL2/adenovirus E1B 19 kd protein interacting protein 3a antibody
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 1/100 ab109414
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109414).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab239976 has not yet been referenced specifically in any publications.