Recombinant
RabMAb

Recombinant Anti-BNIP3L/NIX (phospho S81) antibody [EPR19826] (ab208190)

Overview

  • Product name

    Anti-BNIP3L/NIX (phospho S81) antibody [EPR19826]
    See all BNIP3L/NIX primary antibodies
  • Description

    Rabbit monoclonal [EPR19826] to BNIP3L/NIX (phospho S81)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Dot blot, WBmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    This product was produced with the following immunogens:
    Synthetic peptide within Mouse BNIP3L/NIX aa 50-150 (phospho S81). The exact sequence is proprietary.
    Database link: Q9Z2F7

    Synthetic peptide within Mouse BNIP3L/NIX aa 50-150 (phospho S81). The exact sequence is proprietary.
    Database link: Q9Z2F7

  • Positive control

    • Dot: BNIP3L/NIX (phosphor S81) peptide. WB: HEK-293 transfected with a DDDDK-tagged mouse BNIP3L/NIX (WT) expression vector.
  • General notes

     This product was previously labelled as BNIP3L

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab208190 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot 1/1000.
WB 1/5000. Detects a band of approximately 40, 80 kDa (predicted molecular weight: 24 kDa).

Target

  • Function

    Induces apoptosis. Interacts with viral and cellular anti-apoptosis proteins. Can overcome the suppressors BCL-2 and BCL-XL, although high levels of BCL-XL expression will inhibit apoptosis. May function as a tumor suppressor. Inhibits apoptosis induced by BNIP3.
  • Sequence similarities

    Belongs to the NIP3 family.
  • Post-translational
    modifications

    Undergoes progressive proteolysis to an 11 kDa C-terminal fragment, which is blocked by the proteasome inhibitor lactacystin.
  • Cellular localization

    Nucleus envelope. Endoplasmic reticulum. Mitochondrion. Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Adenovirus E1B19k binding protein B5 antibody
    • Adenovirus E1B19K-binding protein B5 antibody
    • BCL2/adenovirus E1B 19 kd protein interacting protein 3a antibody
    • BCL2/adenovirus E1B 19 kDa protein interacting protein 3A antibody
    • BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like antibody
    • BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A antibody
    • BCL2/adenovirus E1B 19kDa interacting protein 3 like antibody
    • BNI3L_HUMAN antibody
    • BNIP3a antibody
    • BNIP3H antibody
    • BNIP3L antibody
    • BNIP3L protein antibody
    • NIP3 like protein X antibody
    • NIP3-like protein X antibody
    • NIP3L antibody
    • NIX antibody
    see all

Images

  • Dot blot analysis of BNIP3L/NIX (phospho S81) labeled with ab208190 at 1/1000 dilution.

    Lane 1: BNIP3L/NIX (phospho S81) peptide;

    Lane 2: BNIP3L/NIX non-phospho peptide;

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • All lanes : Anti-BNIP3L/NIX (phospho S81) antibody [EPR19826] (ab208190) at 1/5000 dilution

    Lane 1 : HEK-293 (human embryonic kidney) transfected with an empty vector (vector control) containing a DDDDK tag, whole cell lysate
    Lane 2 : HEK-293 transfected with a DDDDK-tagged mouse BNIP3L/NIX (WT) expression vector, whole cell lysate
    Lane 3 : HEK-293 transfected with a DDDDK-tagged mouse BNIP3L/NIX (WT) expression vector, lysate then treated with alkaline phosphatase for 1 hour, whole cell lysate
    Lane 4 : HEK-293 transfected with a DDDDK-tagged mouse BNIP3L/NIX S81A mutant expression vector, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 24 kDa
    Observed band size: 40, 80 kDa
    why is the actual band size different from the predicted?



    The molecular weight observed is consistent with what has been described in the literature (PMID: 10467396).

    The plasmids were kindly provided by our collaborator Dr. Zhong Chen, Zhejiang University.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 seconds.

References

This product has been referenced in:

  • Yuan Y  et al. BNIP3L/NIX-mediated mitophagy protects against ischemic brain injury independent of PARK2. Autophagy N/A:1-13 (2017). Read more (PubMed: 28820284) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
Brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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Verified customer

Submitted Nov 27 2017

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