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    bnp-antibody-50e1-ab20984.pdf

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Anti-BNP antibody [50E1] (ab20984)

  • Datasheet
  • SDS
Submit a review Submit a question References (3)

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Promotion Information

Abpromise

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Key features and details

  • Mouse monoclonal [50E1] to BNP
  • Suitable for: ELISA, ICC/IF, WB, Sandwich ELISA
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-BNP antibody [50E1]
    See all BNP primary antibodies
  • Description

    Mouse monoclonal [50E1] to BNP
  • Host species

    Mouse
  • Specificity

    ab20984 detects Human BNP and proBNP.
  • Tested applications

    Suitable for: ELISA, ICC/IF, WB, Sandwich ELISAmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human BNP conjugated to bovine serum albumin.

  • Epitope

    ab20984 binds to epitope 26-32.
  • General notes

    This product has switched from ascites to TCS on 29th June 2020. Stock up to lot GR3225984 has been generated from ascites.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    50E1
  • Myeloma

    Sp2/0
  • Isotype

    IgG1
  • Research areas

    • Signal Transduction
    • Growth Factors/Hormones
    • Hormones
    • Cardiovascular
    • Blood
    • Blood Pressure regulation
    • Cardiovascular
    • Heart
    • Hypertrophy
    • Hormones
    • Cardiovascular
    • Heart
    • Hypertrophy
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant Human BNP protein (ab87200)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab20984 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration. PubMed: 21482747
WB
Use at an assay dependent concentration.
Sandwich ELISA
Use at an assay dependent concentration.

Can be used as capture antibody

Notes
ELISA
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration. PubMed: 21482747
WB
Use at an assay dependent concentration.
Sandwich ELISA
Use at an assay dependent concentration.

Can be used as capture antibody

Target

  • Relevance

    Brain natriuretic peptide (BNP) circulates in blood as a peptide hormone with natriuretic, vasodilatory and renin inhibitory properties. BNP is secreted predominantly by the left ventricular myocytes in response to volume expansion and pressure overload. BNP belongs to a family of structurally similar peptide hormones, which includes atrial natriuretic peptide (ANP), BNP, C type natriuretic peptide (CNP) and urodilatin. These peptides are characterized by a common 17 amino acid ring structure with a disulfide bond between two cystein residues. This ring structure shows high homology between different natriuretic peptides (eleven of the 17 amino acid residues are homologous in the ring of each of the natriuretic peptides). BNP is a 32 amino acid peptide with disulfide bond between the cysteine residues Cys10 and Cys26. In earlier studies it has been demonstrated that BNP concentration in blood increases with the severity of congestive heart failure. Quantitative measurement of BNP in blood provides an objective indicator of congestive heart failure severity.
  • Cellular localization

    Secreted
  • Database links

    • Entrez Gene: 4879 Human
    • Omim: 600295 Human
    • SwissProt: P16860 Human
    • Alternative names

      • BNP(1-28) antibody
      • BNP(1-29) antibody
      • BNP(1-30) antibody
      • BNP(1-32) antibody
      • BNP(2-31) antibody
      • BNP(3-29) antibody
      • BNP(3-30) antibody
      • BNP(3-32) antibody
      • BNP(4-27) antibody
      • BNP(4-29) antibody
      • BNP(4-30) antibody
      • BNP(4-31) antibody
      • BNP(4-32) antibody
      • BNP(5-29) antibody
      • BNP(5-31) antibody
      • BNP(5-32) antibody
      • Brain natriuretic peptide 32 antibody
      • Brain type natriuretic peptide antibody
      • Gamma brain natriuretic peptide antibody
      • Natriuretic peptide B antibody
      • Natriuretic peptide precursor B antibody
      • natriuretic peptide, brain type antibody
      • Natriuretic peptides B antibody
      • natriuretic protein antibody
      • NPPB antibody
      see all

    Protocols

    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (3)

    Publishing research using ab20984? Please let us know so that we can cite the reference in this datasheet.

    ab20984 has been referenced in 3 publications.

    • Gong Y  et al. Improved LFIAs for highly sensitive detection of BNP at point-of-care. Int J Nanomedicine 12:4455-4466 (2017). PubMed: 28670119
    • Tae HJ  et al. Cardiac remodeling in the mouse model of Marfan syndrome develops into two distinctive phenotypes. Am J Physiol Heart Circ Physiol 310:H290-9 (2016). PubMed: 26566724
    • Tonne JM  et al. Secretion of glycosylated pro-B-type natriuretic peptide from normal cardiomyocytes. Clin Chem 57:864-73 (2011). WB, ICC/IF ; Human . PubMed: 21482747

    Customer reviews and Q&As

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