Anti-BNP antibody [50E1] (ab20984)
- Datasheet
- References (3)
- Protocols
Overview
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Product nameAnti-BNP antibody [50E1]
See all BNP primary antibodies -
DescriptionMouse monoclonal [50E1] to BNP
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Host speciesMouse
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Specificityab20984 detects Human BNP and proBNP.
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Tested applicationsSuitable for: ELISA, Sandwich ELISA, ICC/IF, WBmore details
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Species reactivityReacts with: Human
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Immunogen
Synthetic peptide corresponding to Human BNP conjugated to bovine serum albumin.
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Epitopeab20984 binds to epitope 26-32.
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General notes
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab92500 as a replacement.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4 -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone number50E1
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MyelomaSp2/0
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IsotypeIgG1
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Research areas
Associated products
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Compatible Secondaries
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ELISA pair antibody
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sELISA pair antibody
Applications
Our Abpromise guarantee covers the use of ab20984 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | Use at an assay dependent dilution. Can be paired for ELISA with Mouse monoclonal [16F3] to proBNP (HRP) (ab13124). | |
| Sandwich ELISA | Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Mouse monoclonal [24C5] to BNP (HRP) (ab14699). Can be used as capture antibody | |
| ICC/IF | Use at an assay dependent concentration. PubMed: 21482747 | |
| WB | Use at an assay dependent dilution. Predicted molecular weight: 15 kDa. |
Target
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RelevanceBrain natriuretic peptide (BNP) circulates in blood as a peptide hormone with natriuretic, vasodilatory and renin inhibitory properties. BNP is secreted predominantly by the left ventricular myocytes in response to volume expansion and pressure overload. BNP belongs to a family of structurally similar peptide hormones, which includes atrial natriuretic peptide (ANP), BNP, C type natriuretic peptide (CNP) and urodilatin. These peptides are characterized by a common 17 amino acid ring structure with a disulfide bond between two cystein residues. This ring structure shows high homology between different natriuretic peptides (eleven of the 17 amino acid residues are homologous in the ring of each of the natriuretic peptides). BNP is a 32 amino acid peptide with disulfide bond between the cysteine residues Cys10 and Cys26. In earlier studies it has been demonstrated that BNP concentration in blood increases with the severity of congestive heart failure. Quantitative measurement of BNP in blood provides an objective indicator of congestive heart failure severity.
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Cellular localizationSecreted
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Database links
- Entrez Gene: 4879 Human
- Omim: 600295 Human
- SwissProt: P16860 Human
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Alternative names
- BNP(1-28) antibody
- BNP(1-29) antibody
- BNP(1-30) antibody
see all
References
This product has been referenced in:
- Gong Y et al. Improved LFIAs for highly sensitive detection of BNP at point-of-care. Int J Nanomedicine 12:4455-4466 (2017). Read more (PubMed: 28670119) »
- Tae HJ et al. Cardiac remodeling in the mouse model of Marfan syndrome develops into two distinctive phenotypes. Am J Physiol Heart Circ Physiol 310:H290-9 (2016). Read more (PubMed: 26566724) »
