Anti-BNP antibody [50E1] (ab20984)
Key features and details
- Mouse monoclonal [50E1] to BNP
- Suitable for: ELISA, ICC/IF, WB, Sandwich ELISA
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-BNP antibody [50E1]
See all BNP primary antibodies -
Description
Mouse monoclonal [50E1] to BNP -
Host species
Mouse -
Specificity
ab20984 detects Human BNP and proBNP. -
Tested applications
Suitable for: ELISA, ICC/IF, WB, Sandwich ELISAmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human BNP conjugated to bovine serum albumin.
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Epitope
ab20984 binds to epitope 26-32. -
General notes
This product has switched from ascites to TCS on 29th June 2020. Stock up to lot GR3225984 has been generated from ascites.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
50E1 -
Myeloma
Sp2/0 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab20984 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| ICC/IF |
Use at an assay dependent concentration. PubMed: 21482747
|
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| WB |
Use at an assay dependent concentration.
|
|
| Sandwich ELISA |
Use at an assay dependent concentration.
Can be used as capture antibody |
| Notes |
|---|
|
ELISA
Use at an assay dependent concentration. |
|
ICC/IF
Use at an assay dependent concentration. PubMed: 21482747 |
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WB
Use at an assay dependent concentration. |
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Sandwich ELISA
Use at an assay dependent concentration. Can be used as capture antibody |
Target
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Relevance
Brain natriuretic peptide (BNP) circulates in blood as a peptide hormone with natriuretic, vasodilatory and renin inhibitory properties. BNP is secreted predominantly by the left ventricular myocytes in response to volume expansion and pressure overload. BNP belongs to a family of structurally similar peptide hormones, which includes atrial natriuretic peptide (ANP), BNP, C type natriuretic peptide (CNP) and urodilatin. These peptides are characterized by a common 17 amino acid ring structure with a disulfide bond between two cystein residues. This ring structure shows high homology between different natriuretic peptides (eleven of the 17 amino acid residues are homologous in the ring of each of the natriuretic peptides). BNP is a 32 amino acid peptide with disulfide bond between the cysteine residues Cys10 and Cys26. In earlier studies it has been demonstrated that BNP concentration in blood increases with the severity of congestive heart failure. Quantitative measurement of BNP in blood provides an objective indicator of congestive heart failure severity. -
Cellular localization
Secreted -
Database links
- Entrez Gene: 4879 Human
- Omim: 600295 Human
- SwissProt: P16860 Human
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Alternative names
- BNP(1-28) antibody
- BNP(1-29) antibody
- BNP(1-30) antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab20984 has been referenced in 3 publications.
- Gong Y et al. Improved LFIAs for highly sensitive detection of BNP at point-of-care. Int J Nanomedicine 12:4455-4466 (2017). PubMed: 28670119
- Tae HJ et al. Cardiac remodeling in the mouse model of Marfan syndrome develops into two distinctive phenotypes. Am J Physiol Heart Circ Physiol 310:H290-9 (2016). PubMed: 26566724
- Tonne JM et al. Secretion of glycosylated pro-B-type natriuretic peptide from normal cardiomyocytes. Clin Chem 57:864-73 (2011). WB, ICC/IF ; Human . PubMed: 21482747