Key features and details
- Mouse monoclonal [50E1] to BNP
- Suitable for: ELISA, Sandwich ELISA, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-BNP antibody [50E1]
See all BNP primary antibodies
DescriptionMouse monoclonal [50E1] to BNP
Specificityab20984 detects Human BNP and proBNP.
Tested applicationsSuitable for: ELISA, Sandwich ELISA, ICC/IF, WBmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human BNP conjugated to bovine serum albumin.
Epitopeab20984 binds to epitope 26-32.
This product has switched from ascites to TCS on 29th June 2020. Stock up to lot GR3225984 has been generated from ascites.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Concentration information loading...
PurityProtein A purified
sELISA pair antibody
Our Abpromise guarantee covers the use of ab20984 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent dilution.|
|Sandwich ELISA||Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Mouse monoclonal [24C5] to BNP (HRP) (ab14699). Can be used as capture antibody|
|ICC/IF||Use at an assay dependent concentration. PubMed: 21482747|
|WB||Use at an assay dependent dilution. Predicted molecular weight: 15 kDa.|
RelevanceBrain natriuretic peptide (BNP) circulates in blood as a peptide hormone with natriuretic, vasodilatory and renin inhibitory properties. BNP is secreted predominantly by the left ventricular myocytes in response to volume expansion and pressure overload. BNP belongs to a family of structurally similar peptide hormones, which includes atrial natriuretic peptide (ANP), BNP, C type natriuretic peptide (CNP) and urodilatin. These peptides are characterized by a common 17 amino acid ring structure with a disulfide bond between two cystein residues. This ring structure shows high homology between different natriuretic peptides (eleven of the 17 amino acid residues are homologous in the ring of each of the natriuretic peptides). BNP is a 32 amino acid peptide with disulfide bond between the cysteine residues Cys10 and Cys26. In earlier studies it has been demonstrated that BNP concentration in blood increases with the severity of congestive heart failure. Quantitative measurement of BNP in blood provides an objective indicator of congestive heart failure severity.
- BNP(1-28) antibody
- BNP(1-29) antibody
- BNP(1-30) antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab20984 has been referenced in 3 publications.
- Gong Y et al. Improved LFIAs for highly sensitive detection of BNP at point-of-care. Int J Nanomedicine 12:4455-4466 (2017). PubMed: 28670119
- Tae HJ et al. Cardiac remodeling in the mouse model of Marfan syndrome develops into two distinctive phenotypes. Am J Physiol Heart Circ Physiol 310:H290-9 (2016). PubMed: 26566724
- Tonne JM et al. Secretion of glycosylated pro-B-type natriuretic peptide from normal cardiomyocytes. Clin Chem 57:864-73 (2011). WB, ICC/IF ; Human . PubMed: 21482747