Overview

  • Product name
  • Description
    Rabbit polyclonal to BNP
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC (PFA fixed), RIA, ELISA, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic human Brain Natriuretic Polypeptide covalently attached to a carrier protein (bovine thyroglobulin).

Properties

Applications

Our Abpromise guarantee covers the use of ab19645 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC (PFA fixed) Use at an assay dependent concentration. Has been found to stain 3% buffered paraformaldehyde fixed rat brain sections using indirect immunofluorescent procedure at a dilution of 1/1000.
RIA Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
WB 1/500. Detects a band of approximately 18 kDa (predicted molecular weight: 15 kDa).

Target

  • Relevance
    Brain natriuretic peptide (BNP) circulates in blood as a peptide hormone with natriuretic, vasodilatory and renin inhibitory properties. BNP is secreted predominantly by the left ventricular myocytes in response to volume expansion and pressure overload. BNP belongs to a family of structurally similar peptide hormones, which includes atrial natriuretic peptide (ANP), BNP, C type natriuretic peptide (CNP) and urodilatin. These peptides are characterized by a common 17 amino acid ring structure with a disulfide bond between two cystein residues. This ring structure shows high homology between different natriuretic peptides (eleven of the 17 amino acid residues are homologous in the ring of each of the natriuretic peptides). BNP is a 32 amino acid peptide with disulfide bond between the cysteine residues Cys10 and Cys26. In earlier studies it has been demonstrated that BNP concentration in blood increases with the severity of congestive heart failure. Quantitative measurement of BNP in blood provides an objective indicator of congestive heart failure severity.
  • Cellular localization
    Secreted
  • Database links
  • Alternative names
    • BNP(1-28) antibody
    • BNP(1-29) antibody
    • BNP(1-30) antibody
    • BNP(1-32) antibody
    • BNP(2-31) antibody
    • BNP(3-29) antibody
    • BNP(3-30) antibody
    • BNP(3-32) antibody
    • BNP(4-27) antibody
    • BNP(4-29) antibody
    • BNP(4-30) antibody
    • BNP(4-31) antibody
    • BNP(4-32) antibody
    • BNP(5-29) antibody
    • BNP(5-31) antibody
    • BNP(5-32) antibody
    • Brain natriuretic peptide 32 antibody
    • Brain type natriuretic peptide antibody
    • Gamma brain natriuretic peptide antibody
    • Natriuretic peptide B antibody
    • Natriuretic peptide precursor B antibody
    • natriuretic peptide, brain type antibody
    • Natriuretic peptides B antibody
    • natriuretic protein antibody
    • NPPB antibody
    see all

Images

  • All lanes : Anti-BNP antibody (ab19645) at 1/500 dilution

    Lane 1 : SK N BE (Human neuroblastoma) Whole Cell Lysate
    Lane 2 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
    Lane 3 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size: 15 kDa
    Observed band size: 18 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 40 kDa, 48 kDa, 60 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:
  • Li Y  et al. Aldolase promotes the development of cardiac hypertrophy by targeting AMPK signaling. Exp Cell Res 370:78-86 (2018). Read more (PubMed: 29902536) »
  • Rosales W  et al. Role of Histone Demethylases in Cardiomyocytes Induced to Hypertrophy. Biomed Res Int 2016:2634976 (2016). WB ; Rat . Read more (PubMed: 27722168) »
See all 5 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Abreviews
Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (15% PAGE)
Sample
Mouse Tissue lysate - whole (Hypertrophoid diabetic heart)
Specification
Hypertrophoid diabetic heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Jan 19 2015

Abreviews
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (15% PAGE)
Sample
Mouse Cell lysate - whole cell (P19CL6 cell line)
Specification
P19CL6 cell line
Treatment
a chemical
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Chang Liu

Verified customer

Submitted Oct 08 2014

Answer

Thank you for contacting us regarding ab19645.

I have confirmed with the laboratory that the antibody has only been specifically tested to work with PFA fixed tissue. We do not have any information on how the antibody will work on paraffin embedded tissue or in the presence of different fixatives.

I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

Read More

Answer

Thank you for contacting Abcam regarding ab19645.


I have confirmed that this antibody has not been specifically tested with cardiac tissue in IHC. According to SwissProt, this protein is expressed in the brain, but less so by cells of the atria. If the protein is expressed in your tissue, then the antibody should detect it, but we do not have any data demonstrating this.


I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

Read More

Question
Answer

Thank you for your enquiry. For ab19601, the epitope location has not been determined and the immunogen was full-length BNP peptide. This antibody reacts with both BNP and proBNP so the epitope is NOT located on the NT-proBNP fragment. For ab19645, this is a polyclonal antibody and therefore it will recognize multiple epitopes. The immunogen is full-length BNP peptide and this product will recognize epitopes along the entire BNP molecule. If you have any additional questions, please contact us again.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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