Recombinant Anti-BNP antibody [EPR22339-65] (ab243440)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22339-65] to BNP
- Suitable for: IHC-P
- Reacts with: Mouse, Rat
Overview
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Product name
Anti-BNP antibody [EPR22339-65]
See all BNP primary antibodies -
Description
Rabbit monoclonal [EPR22339-65] to BNP -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details
Unsuitable for: IHC-Fr or WB -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment within Mouse BNP aa 50 to the C-terminus. The exact sequence is proprietary.
Database link: P40753 -
Positive control
- IHC-P: Mouse and rat heart tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22339-65 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab243440 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Relevance
Brain natriuretic peptide (BNP) circulates in blood as a peptide hormone with natriuretic, vasodilatory and renin inhibitory properties. BNP is secreted predominantly by the left ventricular myocytes in response to volume expansion and pressure overload. BNP belongs to a family of structurally similar peptide hormones, which includes atrial natriuretic peptide (ANP), BNP, C type natriuretic peptide (CNP) and urodilatin. These peptides are characterized by a common 17 amino acid ring structure with a disulfide bond between two cystein residues. This ring structure shows high homology between different natriuretic peptides (eleven of the 17 amino acid residues are homologous in the ring of each of the natriuretic peptides). BNP is a 32 amino acid peptide with disulfide bond between the cysteine residues Cys10 and Cys26. In earlier studies it has been demonstrated that BNP concentration in blood increases with the severity of congestive heart failure. Quantitative measurement of BNP in blood provides an objective indicator of congestive heart failure severity. -
Cellular localization
Secreted -
Database links
- Entrez Gene: 18158 Mouse
- Entrez Gene: 25105 Rat
- SwissProt: P40753 Mouse
- SwissProt: P13205 Rat
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Alternative names
- BNP(1-28) antibody
- BNP(1-29) antibody
- BNP(1-30) antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNP antibody [EPR22339-65] (ab243440)](https://www.abcam.com/ps/products/243/ab243440/Images/ab243440-334085-anti-bnp-antibody-epr22339-65-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNP antibody [EPR22339-65] (ab243440)Immunohistochemical analysis of paraffin-embedded mouse heart tissue labeling BNP with ab243440 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on the atrium (PMID: 15576492). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNP antibody [EPR22339-65] (ab243440)](https://www.abcam.com/ps/products/243/ab243440/Images/ab243440-334086-anti-bnp-antibody-epr22339-65-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNP antibody [EPR22339-65] (ab243440)Immunohistochemical analysis of paraffin-embedded rat heart tissue labeling BNP with ab243440 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on the atrium (PMID: 15576492). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNP antibody [EPR22339-65] (ab243440)](https://www.abcam.com/ps/products/243/ab243440/Images/ab243440-334087-anti-bnp-antibody-epr22339-65-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNP antibody [EPR22339-65] (ab243440)Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling BNP with ab243440 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Counter stained with hematoxylin.
Negative tissue: no staining on mouse liver (PMID: 15576492).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNP antibody [EPR22339-65] (ab243440)](https://www.abcam.com/ps/products/243/ab243440/Images/ab243440-334088-anti-bnp-antibody-epr22339-65-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNP antibody [EPR22339-65] (ab243440)Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling BNP with ab243440 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Counter stained with hematoxylin.
Negative tissue: no staining on rat liver (PMID: 15576492).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Anti-BNP antibody [EPR22339-65] (ab243440)](https://www.abcam.com/ps/products/243/ab243440/Images/ab243440-5-benefits-of-recombinant-antibodies.png)
Anti-BNP antibody [EPR22339-65] (ab243440)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab243440 has not yet been referenced specifically in any publications.
