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    bnp-antibody-epr22339-65-ab243440.pdf

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RecombinantRabMAb

Recombinant Anti-BNP antibody [EPR22339-65] (ab243440)

  • Datasheet
  • SDS
  • Certificate of Compliance
Submit a review Submit a question References (1)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNP antibody [EPR22339-65] (ab243440)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNP antibody [EPR22339-65] (ab243440)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNP antibody [EPR22339-65] (ab243440)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNP antibody [EPR22339-65] (ab243440)
  • Anti-BNP antibody [EPR22339-65] (ab243440)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22339-65] to BNP
  • Suitable for: IHC-P
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Carrier Free

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Overview

  • Product name

    Anti-BNP antibody [EPR22339-65]
    See all BNP primary antibodies
  • Description

    Rabbit monoclonal [EPR22339-65] to BNP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
    Unsuitable for: IHC-Fr or WB
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse and rat heart tissues.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR22339-65
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Growth Factors/Hormones
    • Hormones
    • Cardiovascular
    • Blood
    • Blood Pressure regulation
    • Cardiovascular
    • Heart
    • Hypertrophy
    • Hormones
    • Cardiovascular
    • Heart
    • Hypertrophy
    • Other

Associated products

  • Alternative Versions

    • Anti-BNP antibody [EPR22339-65] - BSA and Azide free (ab243857)
  • Recombinant Protein

    • Recombinant Human BNP protein (ab87200)
  • Related Products

    • Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab243440 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Application notes
Is unsuitable for IHC-Fr or WB.

Target

  • Relevance

    Brain natriuretic peptide (BNP) circulates in blood as a peptide hormone with natriuretic, vasodilatory and renin inhibitory properties. BNP is secreted predominantly by the left ventricular myocytes in response to volume expansion and pressure overload. BNP belongs to a family of structurally similar peptide hormones, which includes atrial natriuretic peptide (ANP), BNP, C type natriuretic peptide (CNP) and urodilatin. These peptides are characterized by a common 17 amino acid ring structure with a disulfide bond between two cystein residues. This ring structure shows high homology between different natriuretic peptides (eleven of the 17 amino acid residues are homologous in the ring of each of the natriuretic peptides). BNP is a 32 amino acid peptide with disulfide bond between the cysteine residues Cys10 and Cys26. In earlier studies it has been demonstrated that BNP concentration in blood increases with the severity of congestive heart failure. Quantitative measurement of BNP in blood provides an objective indicator of congestive heart failure severity.
  • Cellular localization

    Secreted
  • Database links

    • Entrez Gene: 18158 Mouse
    • Entrez Gene: 25105 Rat
    • SwissProt: P40753 Mouse
    • SwissProt: P13205 Rat
    • Alternative names

      • BNP(1-28) antibody
      • BNP(1-29) antibody
      • BNP(1-30) antibody
      • BNP(1-32) antibody
      • BNP(2-31) antibody
      • BNP(3-29) antibody
      • BNP(3-30) antibody
      • BNP(3-32) antibody
      • BNP(4-27) antibody
      • BNP(4-29) antibody
      • BNP(4-30) antibody
      • BNP(4-31) antibody
      • BNP(4-32) antibody
      • BNP(5-29) antibody
      • BNP(5-31) antibody
      • BNP(5-32) antibody
      • Brain natriuretic peptide 32 antibody
      • Brain type natriuretic peptide antibody
      • Gamma brain natriuretic peptide antibody
      • Natriuretic peptide B antibody
      • Natriuretic peptide precursor B antibody
      • natriuretic peptide, brain type antibody
      • Natriuretic peptides B antibody
      • natriuretic protein antibody
      • NPPB antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNP antibody [EPR22339-65] (ab243440)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNP antibody [EPR22339-65] (ab243440)

      Immunohistochemical analysis of paraffin-embedded mouse heart tissue labeling BNP with ab243440 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on the atrium (PMID: 15576492). Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNP antibody [EPR22339-65] (ab243440)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNP antibody [EPR22339-65] (ab243440)

      Immunohistochemical analysis of paraffin-embedded rat heart tissue labeling BNP with ab243440 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on the atrium (PMID: 15576492). Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNP antibody [EPR22339-65] (ab243440)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNP antibody [EPR22339-65] (ab243440)

      Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling BNP with ab243440 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Counter stained with hematoxylin.

      Negative tissue: no staining on mouse liver (PMID: 15576492).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNP antibody [EPR22339-65] (ab243440)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNP antibody [EPR22339-65] (ab243440)

      Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling BNP with ab243440 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Counter stained with hematoxylin.

      Negative tissue: no staining on rat liver (PMID: 15576492).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Anti-BNP antibody [EPR22339-65] (ab243440)
      Anti-BNP antibody [EPR22339-65] (ab243440)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (1)

    Publishing research using ab243440? Please let us know so that we can cite the reference in this datasheet.

    ab243440 has been referenced in 1 publication.

    • Cheng Z  et al. lncRNA ZEB2-AS1 stimulates cardiac hypertrophy by downregulating PTEN. Exp Ther Med 20:92 (2020). PubMed: 32973941

    Customer reviews and Q&As

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