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RecombinantRabMAb

Recombinant Anti-BNP antibody [EPR3735] - BSA and Azide free (ab247572)

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Immunocytochemistry - Anti-BNP antibody [EPR3735] - BSA and Azide free (ab247572)
  • Western blot - Anti-BNP antibody [EPR3735] - BSA and Azide free (ab247572)
  • Flow Cytometry - Anti-BNP antibody [EPR3735] - BSA and Azide free (ab247572)
  • Anti-BNP antibody [EPR3735] - BSA and Azide free (ab247572)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3735] to BNP - BSA and Azide free
  • Suitable for: Flow Cyt, ICC, WB
  • Reacts with: Human

You may also be interested in

Conjugation
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HRP Conjugation Kit - Lightning-Link® (ab102890)
Protein
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Recombinant Human BNP protein (ab87200)

View more associated products

Overview

  • Product name

    Anti-BNP antibody [EPR3735] - BSA and Azide free
    See all BNP primary antibodies
  • Description

    Rabbit monoclonal [EPR3735] to BNP - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, ICC, WBmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human BNP aa 50-150. The exact sequence is proprietary.

  • Positive control

    • WB: Human plasma and fetal heart lysate; ICC: Caco-2 cells; Flow Cyt: Caco-2 cells.
  • General notes

    ab247572 is the carrier-free version of ab92500 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab247572 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR3735
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Growth Factors/Hormones
    • Hormones
    • Cardiovascular
    • Blood
    • Blood Pressure regulation
    • Cardiovascular
    • Heart
    • Hypertrophy
    • Hormones
    • Cardiovascular
    • Heart
    • Hypertrophy
    • Other

Associated products

  • Alternative Versions

    • Anti-BNP antibody [EPR3735] (ab92500)
  • Conjugation kits

    • HRP Conjugation Kit - Lightning-Link® (ab102890)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)
  • Recombinant Protein

    • Recombinant Human BNP protein (ab87200)

Applications

Our Abpromise guarantee covers the use of ab247572 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 30 kDa (predicted molecular weight: 15 kDa).
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Relevance

      Brain natriuretic peptide (BNP) circulates in blood as a peptide hormone with natriuretic, vasodilatory and renin inhibitory properties. BNP is secreted predominantly by the left ventricular myocytes in response to volume expansion and pressure overload. BNP belongs to a family of structurally similar peptide hormones, which includes atrial natriuretic peptide (ANP), BNP, C type natriuretic peptide (CNP) and urodilatin. These peptides are characterized by a common 17 amino acid ring structure with a disulfide bond between two cystein residues. This ring structure shows high homology between different natriuretic peptides (eleven of the 17 amino acid residues are homologous in the ring of each of the natriuretic peptides). BNP is a 32 amino acid peptide with disulfide bond between the cysteine residues Cys10 and Cys26. In earlier studies it has been demonstrated that BNP concentration in blood increases with the severity of congestive heart failure. Quantitative measurement of BNP in blood provides an objective indicator of congestive heart failure severity.
    • Cellular localization

      Secreted
    • Database links

      • Entrez Gene: 4879 Human
      • Omim: 600295 Human
      • SwissProt: P16860 Human
      • Alternative names

        • BNP(1-28) antibody
        • BNP(1-29) antibody
        • BNP(1-30) antibody
        • BNP(1-32) antibody
        • BNP(2-31) antibody
        • BNP(3-29) antibody
        • BNP(3-30) antibody
        • BNP(3-32) antibody
        • BNP(4-27) antibody
        • BNP(4-29) antibody
        • BNP(4-30) antibody
        • BNP(4-31) antibody
        • BNP(4-32) antibody
        • BNP(5-29) antibody
        • BNP(5-31) antibody
        • BNP(5-32) antibody
        • Brain natriuretic peptide 32 antibody
        • Brain type natriuretic peptide antibody
        • Gamma brain natriuretic peptide antibody
        • Natriuretic peptide B antibody
        • Natriuretic peptide precursor B antibody
        • natriuretic peptide, brain type antibody
        • Natriuretic peptides B antibody
        • natriuretic protein antibody
        • NPPB antibody
        see all

      Images

      • Immunocytochemistry - Anti-BNP antibody [EPR3735] - BSA and Azide free (ab247572)
        Immunocytochemistry - Anti-BNP antibody [EPR3735] - BSA and Azide free (ab247572)

        This data was developed using ab92500, the same antibody clone in a different buffer formulation.

        Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Caco-2 cells labelling BNP with ab92500 at 1/50 dilution (10.8 µg/mL), followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing cytoplamic staining in Caco-2 cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 µg/mL) (Red). The Nuclear counterstain was DAPI (Blue).

        Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (2 µg/mL).

      • Western blot - Anti-BNP antibody [EPR3735] - BSA and Azide free (ab247572)
        Western blot - Anti-BNP antibody [EPR3735] - BSA and Azide free (ab247572)
        All lanes : Anti-BNP antibody [EPR3735] (ab92500) at 1/2000 dilution

        Lane 1 : Human plasma
        Lane 2 : Human fetal heart lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : goat anti-rabbit HRP at 1/2000 dilution

        Predicted band size: 15 kDa
        Observed band size: 30 kDa
        why is the actual band size different from the predicted?



        This data was developed using ab92500, the same antibody clone in a different buffer formulation.

         

      • Flow Cytometry - Anti-BNP antibody [EPR3735] - BSA and Azide free (ab247572)
        Flow Cytometry - Anti-BNP antibody [EPR3735] - BSA and Azide free (ab247572)

        This data was developed using ab92500, the same antibody clone in a different buffer formulation.

        Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Caco-2 (Human colorectal adenocarcinoma epithelial cell) cells labelling BNP with ab92500 at 1/50 dilution (1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
      • Anti-BNP antibody [EPR3735] - BSA and Azide free (ab247572)
        Anti-BNP antibody [EPR3735] - BSA and Azide free (ab247572)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet

      Certificate of Compliance

      To download a Certificate of Compliance, please enter your Lot number below:

    • References (0)

      Publishing research using ab247572? Please let us know so that we can cite the reference in this datasheet.

      ab247572 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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