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RecombinantRabMAb

Recombinant Anti-BNP antibody [EPR3735] - BSA and Azide free (ab247572)

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Immunocytochemistry - Anti-BNP antibody [EPR3735] - BSA and Azide free (ab247572)
  • Western blot - Anti-BNP antibody [EPR3735] - BSA and Azide free (ab247572)
  • Flow Cytometry (Intracellular) - Anti-BNP antibody [EPR3735] - BSA and Azide free (ab247572)
  • Anti-BNP antibody [EPR3735] - BSA and Azide free (ab247572)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3735] to BNP - BSA and Azide free
  • Suitable for: ICC, WB, Flow Cyt (Intra)
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-BNP antibody [EPR3735] - BSA and Azide free
    See all BNP primary antibodies

  • Description

    Rabbit monoclonal [EPR3735] to BNP - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, WB, Flow Cyt (Intra)more details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human plasma and fetal heart lysate; ICC: Caco-2 cells; Flow Cyt (intra): Caco-2 cells.

  • General notes

    ab247572 is the carrier-free version of ab92500.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab247572 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 30 kDa (predicted molecular weight: 15 kDa).
Flow Cyt (Intra)
Use at an assay dependent concentration.
Notes
ICC
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 30 kDa (predicted molecular weight: 15 kDa).
Flow Cyt (Intra)
Use at an assay dependent concentration.
Application notes
Is unsuitable for IHC-P or IP.

Target

  • Relevance

    Brain natriuretic peptide (BNP) circulates in blood as a peptide hormone with natriuretic, vasodilatory and renin inhibitory properties. BNP is secreted predominantly by the left ventricular myocytes in response to volume expansion and pressure overload. BNP belongs to a family of structurally similar peptide hormones, which includes atrial natriuretic peptide (ANP), BNP, C type natriuretic peptide (CNP) and urodilatin. These peptides are characterized by a common 17 amino acid ring structure with a disulfide bond between two cystein residues. This ring structure shows high homology between different natriuretic peptides (eleven of the 17 amino acid residues are homologous in the ring of each of the natriuretic peptides). BNP is a 32 amino acid peptide with disulfide bond between the cysteine residues Cys10 and Cys26. In earlier studies it has been demonstrated that BNP concentration in blood increases with the severity of congestive heart failure. Quantitative measurement of BNP in blood provides an objective indicator of congestive heart failure severity.

  • Cellular localization

    Secreted

  • Database links

    • Alternative names

      • BNP(1-28) antibody
      • BNP(1-29) antibody
      • BNP(1-30) antibody
      • BNP(1-32) antibody
      • BNP(2-31) antibody
      • BNP(3-29) antibody
      • BNP(3-30) antibody
      • BNP(3-32) antibody
      • BNP(4-27) antibody
      • BNP(4-29) antibody
      • BNP(4-30) antibody
      • BNP(4-31) antibody
      • BNP(4-32) antibody
      • BNP(5-29) antibody
      • BNP(5-31) antibody
      • BNP(5-32) antibody
      • Brain natriuretic peptide 32 antibody
      • Brain type natriuretic peptide antibody
      • Gamma brain natriuretic peptide antibody
      • Natriuretic peptide B antibody
      • Natriuretic peptide precursor B antibody
      • natriuretic peptide, brain type antibody
      • Natriuretic peptides B antibody
      • natriuretic protein antibody
      • NPPB antibody
      see all

    Images

    • Immunocytochemistry - Anti-BNP antibody [EPR3735] - BSA and Azide free (ab247572)
      Immunocytochemistry - Anti-BNP antibody [EPR3735] - BSA and Azide free (ab247572)

      This data was developed using ab92500, the same antibody clone in a different buffer formulation.

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Caco-2 cells labelling BNP with ab92500 at 1/50 dilution (10.8 µg/mL), followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing cytoplamic staining in Caco-2 cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 µg/mL) (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (2 µg/mL).

    • Western blot - Anti-BNP antibody [EPR3735] - BSA and Azide free (ab247572)
      Western blot - Anti-BNP antibody [EPR3735] - BSA and Azide free (ab247572)
      All lanes : Anti-BNP antibody [EPR3735] (ab92500) at 1/2000 dilution

      Lane 1 : Human plasma
      Lane 2 : Human fetal heart lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 15 kDa
      Observed band size: 30 kDa why is the actual band size different from the predicted?



      This data was developed using ab92500, the same antibody clone in a different buffer formulation.

       

    • Flow Cytometry (Intracellular) - Anti-BNP antibody [EPR3735] - BSA and Azide free (ab247572)
      Flow Cytometry (Intracellular) - Anti-BNP antibody [EPR3735] - BSA and Azide free (ab247572)

      This data was developed using ab92500, the same antibody clone in a different buffer formulation. 

      Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Caco-2 (Human colorectal adenocarcinoma epithelial cell) cells labelling BNP with ab92500 at 1/50 dilution (1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

       

    • Anti-BNP antibody [EPR3735] - BSA and Azide free (ab247572)
      Anti-BNP antibody [EPR3735] - BSA and Azide free (ab247572)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab247572? Please let us know so that we can cite the reference in this datasheet.

    ab247572 has not yet been referenced specifically in any publications.

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