Anti-BOB1 antibody [BOB1/2425] (ab238036)
![Protein Array - Anti-BOB1 antibody [BOB1/2425] (ab238036)](https://www.abcam.com/ps/products/238/ab238036/Images/ab238036-3-ab238036.jpg "Protein Array - Anti-BOB1 antibody [BOB1/2425] (ab238036)")
Key features and details
- Mouse monoclonal [BOB1/2425] to BOB1
- Suitable for: Protein Array, WB, IHC-P
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-BOB1 antibody [BOB1/2425]
See all BOB1 primary antibodies -
Description
Mouse monoclonal [BOB1/2425] to BOB1 -
Host species
Mouse -
Tested applications
Suitable for: Protein Array, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human BOB1 aa 148-255. The exact sequence is proprietary.
Database link: Q16633 -
Positive control
- WB: Raji cell lysate. IHC-P: Human spleen tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
Purified from Bioreactor Concentrate by Protein A/G. -
Clonality
Monoclonal -
Clone number
BOB1/2425 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab238036 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Protein Array |
Use at an assay dependent concentration.
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| WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 27 kDa.
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| IHC-P |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Incubate with primary antibody for 30 minutes at room temperature. |
| Notes |
|---|
|
Protein Array
Use at an assay dependent concentration. |
|
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 27 kDa. |
|
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Incubate with primary antibody for 30 minutes at room temperature. |
Target
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Function
Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers. -
Tissue specificity
B-cell specific. -
Involvement in disease
Note=A chromosomal aberration involving POU2AF1/OBF1 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with BCL6. -
Sequence similarities
Belongs to the POU2AF1 family. -
Post-translational
modificationsUbiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5450 Human
- Omim: 601206 Human
- SwissProt: Q16633 Human
- Unigene: 654525 Human
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Alternative names
- B cell Oct binding protein 1 antibody
- B cell specific coactivator OBF 1 antibody
- B cell specific coactivator OBF1 antibody
see all
Images
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Protein Array - Anti-BOB1 antibody [BOB1/2425] (ab238036)ab238036 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29. -
![Western blot - Anti-BOB1 antibody [BOB1/2425] (ab238036)](https://www.abcam.com/ps/products/238/ab238036/Images/ab238036-315771-83b.jpg)
Western blot - Anti-BOB1 antibody [BOB1/2425] (ab238036)Anti-BOB1 antibody [BOB1/2425] (ab238036) at 2 µg/ml + Raji (human Burkitt's lymphoma cell line) cell lysate
Predicted band size: 27 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOB1 antibody [BOB1/2425] (ab238036)](https://www.abcam.com/ps/products/238/ab238036/Images/ab238036-315772-83a.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOB1 antibody [BOB1/2425] (ab238036)Formalin-fixed, paraffin-embedded human spleen tissue stained for BOB1 using ab238036 at 2 μg/ml in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab238036 has been referenced in 1 publication.
- McDonough JE et al. Transcriptional regulatory model of fibrosis progression in the human lung. JCI Insight 4:N/A (2019). PubMed: 31600171
