Anti-BOB1 antibody [BOB1/2425] (ab238036)

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Key features and details

  • Mouse monoclonal [BOB1/2425] to BOB1
  • Suitable for: Protein Array, WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG2b

Overview

  • Product name

    Anti-BOB1 antibody [BOB1/2425]
    See all BOB1 primary antibodies

  • Description

    Mouse monoclonal [BOB1/2425] to BOB1

  • Host species

    Mouse

  • Tested applications

    Suitable for: Protein Array, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human BOB1 aa 148-255. The exact sequence is proprietary.
    Database link: Q16633

  • Positive control

    • WB: Raji cell lysate. IHC-P: Human spleen tissue.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab238036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Protein Array Use at an assay dependent concentration.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 27 kDa.
IHC-P Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Incubate with primary antibody for 30 minutes at room temperature.

Target

  • Function

    Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.

  • Tissue specificity

    B-cell specific.

  • Involvement in disease

    Note=A chromosomal aberration involving POU2AF1/OBF1 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with BCL6.

  • Sequence similarities

    Belongs to the POU2AF1 family.

  • Post-translational
    modifications

    Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • B cell Oct binding protein 1 antibody
      • B cell specific coactivator OBF 1 antibody
      • B cell specific coactivator OBF1 antibody
      • B-cell-specific coactivator OBF-1 antibody
      • BOB 1 antibody
      • BOB-1 antibody
      • OBF 1 antibody
      • OBF1 antibody
      • OBF1_HUMAN antibody
      • OCA B antibody
      • OCA-B antibody
      • OCAB antibody
      • OCT binding factor 1 antibody
      • OCT-binding factor 1 antibody
      • POU class 2 associating factor 1 antibody
      • POU domain class 2 associating factor 1 antibody
      • POU domain class 2-associating factor 1 antibody
      • Pou2af1 antibody
      see all

    Images

    • Protein Array - Anti-BOB1 antibody [BOB1/2425] (ab238036)
      Protein Array - Anti-BOB1 antibody [BOB1/2425] (ab238036)
      ab238036 was tested in protein array against over 19000 different full-length human proteins.
      Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
      A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
    • Western blot - Anti-BOB1 antibody [BOB1/2425] (ab238036)
      Western blot - Anti-BOB1 antibody [BOB1/2425] (ab238036)
      Anti-BOB1 antibody [BOB1/2425] (ab238036) at 2 µg/ml + Raji (human Burkitt's lymphoma cell line) cell lysate

      Predicted band size: 27 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOB1 antibody [BOB1/2425] (ab238036)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOB1 antibody [BOB1/2425] (ab238036)

      Formalin-fixed, paraffin-embedded human spleen tissue stained for BOB1 using ab238036 at 2 μg/ml in immunohistochemical analysis.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab238036? Please let us know so that we can cite the reference in this datasheet.

    ab238036 has been referenced in 1 publication.

    • McDonough JE  et al. Transcriptional regulatory model of fibrosis progression in the human lung. JCI Insight 4:N/A (2019). PubMed: 31600171

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