Overview

  • Product name

    Anti-BOB1 antibody - C-terminal
    See all BOB1 primary antibodies
  • Description

    Goat polyclonal to BOB1 - C-terminal
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Cow, Dog, Chimpanzee, Monkey, Gorilla, Common marmoset, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human BOB1 aa 241-254 (C terminal) (Cysteine residue). (NP_006226.2).
    Sequence:

    DSDAYALNHTLSVE


    Database link: Q16633

  • Positive control

    • Human spleen tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab189375 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 27 kDa.

In transfected HEK293 transiently expressing full-length Human POU2AF1 (myc and DYKDDDDK tagged), a band of approx. 38kD was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody.

IHC-P Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.
  • Tissue specificity

    B-cell specific.
  • Involvement in disease

    Note=A chromosomal aberration involving POU2AF1/OBF1 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with BCL6.
  • Sequence similarities

    Belongs to the POU2AF1 family.
  • Post-translational
    modifications

    Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • B cell Oct binding protein 1 antibody
    • B cell specific coactivator OBF 1 antibody
    • B cell specific coactivator OBF1 antibody
    • B-cell-specific coactivator OBF-1 antibody
    • BOB 1 antibody
    • BOB-1 antibody
    • OBF 1 antibody
    • OBF1 antibody
    • OBF1_HUMAN antibody
    • OCA B antibody
    • OCA-B antibody
    • OCAB antibody
    • OCT binding factor 1 antibody
    • OCT-binding factor 1 antibody
    • POU class 2 associating factor 1 antibody
    • POU domain class 2 associating factor 1 antibody
    • POU domain class 2-associating factor 1 antibody
    • Pou2af1 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human spleen tissue labelling BOB1 with ab189375 at 3.75 µg/ml.

References

ab189375 has not yet been referenced specifically in any publications.

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