Recombinant
RabMAb

Recombinant Anti-BOB1 antibody [EPR17685] (ab203110)

Overview

  • Product name
    Anti-BOB1 antibody [EPR17685]
    See all BOB1 primary antibodies
  • Description
    Rabbit monoclonal [EPR17685] to BOB1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human BOB1 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q16633

  • Positive control
    • WB: RAMOS, Raji, Human lymph node or Human bone marrow whole cell lysate. IHC: Human Non-Hodgkin lymphoma of B- lineage or Human colon tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab203110 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 35 kDa (predicted molecular weight: 27 kDa).
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function
    Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.
  • Tissue specificity
    B-cell specific.
  • Involvement in disease
    Note=A chromosomal aberration involving POU2AF1/OBF1 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with BCL6.
  • Sequence similarities
    Belongs to the POU2AF1 family.
  • Post-translational
    modifications
    Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • B cell Oct binding protein 1 antibody
    • B cell specific coactivator OBF 1 antibody
    • B cell specific coactivator OBF1 antibody
    • B-cell-specific coactivator OBF-1 antibody
    • BOB 1 antibody
    • BOB-1 antibody
    • OBF 1 antibody
    • OBF1 antibody
    • OBF1_HUMAN antibody
    • OCA B antibody
    • OCA-B antibody
    • OCAB antibody
    • OCT binding factor 1 antibody
    • OCT-binding factor 1 antibody
    • POU class 2 associating factor 1 antibody
    • POU domain class 2 associating factor 1 antibody
    • POU domain class 2-associating factor 1 antibody
    • Pou2af1 antibody
    see all

Images

  • Anti-BOB1 antibody [EPR17685] (ab203110) at 1/10000 dilution + RAMOS (Human Burkitt's lymphoma cell line) whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 27 kDa
    Observed band size: 35 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

     

    The MW profile observed is consistent with what has been described in the literature PMID: 12857960.

  • Anti-BOB1 antibody [EPR17685] (ab203110) at 1/5000 dilution + Raji (Human Burkitt's lymphoma cell line) whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 27 kDa
    Observed band size: 35 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/dilution buffer 5% NFDM/TBST.

     

    The MW profile observed is consistent with what has been described in the literature PMID: 12857960.

  • All lanes : Anti-BOB1 antibody [EPR17685] (ab203110) at 1/200 dilution

    Lane 1 : Human lymph node whole cell lysate
    Lane 2 : Human bone marrow whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/50000 dilution

    Predicted band size: 27 kDa
    Observed band size: 35 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST. 

     

    The MW profile observed is consistent with what has been described in the literature PMID: 12857960.

  • Immunohistochemical analysis of paraffin-embedded Human Non-Hodgkin lymphoma of B- lineage tissue labeling BOB1 Protein with ab230110 at 1/500 dilution followed by ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody at a 1/500 dilution. Nuclear and weak cytoplasmic staining on Human non-hodgkin lymphoma of B- lineage tissue is observed. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is ab97051 Goat Anti-Rabbit IgG H&L (HRP).

     

    Reference: Greiner A et al. Up-Regulation of BOB.1/OBF.1 Expression in Normal Germinal Center B Cells and Germinal Center-Derived Lymphomas. Am J Pathol. 2000 February; 156(2): 501–507.

  • Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling BOB1 Protein with ab230110 at 1/500 dilution followed by ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody at a 1/500 dilution. Nuclear and weakly cytoplasmic staining on lymphocyte of Human colon lineage tissue is observed. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is ab97051 Goat Anti-Rabbit IgG H&L (HRP).

     

    Reference: Greiner A et al. Up-Regulation of BOB.1/OBF.1 Expression in Normal Germinal Center B Cells and Germinal Center-Derived Lymphomas. Am J Pathol. 2000 February; 156(2): 501–507.

  • Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling BOB1 Protein with ab230110 at 1/500 dilution followed by ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody at a 1/500 dilution. Counter stained with Hematoxylin. No staining on Human cerebral cortex tissue (BOB1 Negative tissue)  is observed.

    Negative control: Used PBS instead of primary antibody, secondary antibody is ab97051 Goat Anti-Rabbit IgG H&L (HRP).

     

    Reference: Greiner A et al. Up-Regulation of BOB.1/OBF.1 Expression in Normal Germinal Center B Cells and Germinal Center-Derived Lymphomas. Am J Pathol. 2000 February; 156(2): 501–507.

References

ab203110 has not yet been referenced specifically in any publications.

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