Recombinant Anti-BOB1 antibody [EPR17685] (ab203110)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17685] to BOB1
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-BOB1 antibody [EPR17685]
See all BOB1 primary antibodies -
Description
Rabbit monoclonal [EPR17685] to BOB1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: RAMOS, Raji, Human lymph node or Human bone marrow whole cell lysate. IHC: Human Non-Hodgkin lymphoma of B- lineage or Human colon tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17685 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab203110 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/5000. Detects a band of approximately 35 kDa (predicted molecular weight: 27 kDa).
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IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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WB
1/5000. Detects a band of approximately 35 kDa (predicted molecular weight: 27 kDa). |
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers. -
Tissue specificity
B-cell specific. -
Involvement in disease
Note=A chromosomal aberration involving POU2AF1/OBF1 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with BCL6. -
Sequence similarities
Belongs to the POU2AF1 family. -
Post-translational
modificationsUbiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5450 Human
- Omim: 601206 Human
- SwissProt: Q16633 Human
- Unigene: 654525 Human
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Alternative names
- B cell Oct binding protein 1 antibody
- B cell specific coactivator OBF 1 antibody
- B cell specific coactivator OBF1 antibody
see all
Images
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Anti-BOB1 antibody [EPR17685] (ab203110) at 1/10000 dilution + RAMOS (Human Burkitt's lymphoma cell line) whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 27 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The MW profile observed is consistent with what has been described in the literature PMID: 12857960.
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Anti-BOB1 antibody [EPR17685] (ab203110) at 1/5000 dilution + Raji (Human Burkitt's lymphoma cell line) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 27 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/dilution buffer 5% NFDM/TBST.
The MW profile observed is consistent with what has been described in the literature PMID: 12857960.
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All lanes : Anti-BOB1 antibody [EPR17685] (ab203110) at 1/200 dilution
Lane 1 : Human lymph node whole cell lysate
Lane 2 : Human bone marrow whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/50000 dilution
Predicted band size: 27 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
The MW profile observed is consistent with what has been described in the literature PMID: 12857960.
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Immunohistochemical analysis of paraffin-embedded Human Non-Hodgkin lymphoma of B- lineage tissue labeling BOB1 Protein with ab230110 at 1/500 dilution followed by ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody at a 1/500 dilution. Nuclear and weak cytoplasmic staining on Human non-hodgkin lymphoma of B- lineage tissue is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is ab97051 Goat Anti-Rabbit IgG H&L (HRP).
Reference: Greiner A et al. Up-Regulation of BOB.1/OBF.1 Expression in Normal Germinal Center B Cells and Germinal Center-Derived Lymphomas. Am J Pathol. 2000 February; 156(2): 501–507.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling BOB1 Protein with ab230110 at 1/500 dilution followed by ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody at a 1/500 dilution. Nuclear and weakly cytoplasmic staining on lymphocyte of Human colon lineage tissue is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is ab97051 Goat Anti-Rabbit IgG H&L (HRP).
Reference: Greiner A et al. Up-Regulation of BOB.1/OBF.1 Expression in Normal Germinal Center B Cells and Germinal Center-Derived Lymphomas. Am J Pathol. 2000 February; 156(2): 501–507.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling BOB1 Protein with ab230110 at 1/500 dilution followed by ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody at a 1/500 dilution. Counter stained with Hematoxylin. No staining on Human cerebral cortex tissue (BOB1 Negative tissue) is observed.
Negative control: Used PBS instead of primary antibody, secondary antibody is ab97051 Goat Anti-Rabbit IgG H&L (HRP).
Reference: Greiner A et al. Up-Regulation of BOB.1/OBF.1 Expression in Normal Germinal Center B Cells and Germinal Center-Derived Lymphomas. Am J Pathol. 2000 February; 156(2): 501–507.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab203110 has not yet been referenced specifically in any publications.