Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3299] to BOB1
- Suitable for: WB, IP, Flow Cyt
- Reacts with: Human
Product nameAnti-BOB1 antibody [EPR3299]
See all BOB1 primary antibodies
DescriptionRabbit monoclonal [EPR3299] to BOB1
Tested applicationsSuitable for: WB, IP, Flow Cytmore details
Unsuitable for: ICC or IHC-P
Species reactivityReacts with: Human
Synthetic peptide within Human BOB1 aa 50-150. The exact sequence is proprietary.
- Ramos, Daudi, NAMALWA or Raji nuclear lysate
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab92315 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 28 kDa.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionTranscriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.
Tissue specificityB-cell specific.
Involvement in diseaseNote=A chromosomal aberration involving POU2AF1/OBF1 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with BCL6.
Sequence similaritiesBelongs to the POU2AF1 family.
modificationsUbiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
- Information by UniProt
- B cell Oct binding protein 1 antibody
- B cell specific coactivator OBF 1 antibody
- B cell specific coactivator OBF1 antibody
All lanes : Anti-BOB1 antibody [EPR3299] (ab92315) at 1/2000 dilution
Lane 1 : Ramos cell lysate
Lane 2 : Daudi cell lysate
Lane 3 : NAMALWA cell lysate
Lane 4 : Raji cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 28 kDa
ab92315 has not yet been referenced specifically in any publications.