Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP92] to BOB1
- Suitable for: WB, IHC-P
- Reacts with: Human
Product nameAnti-BOB1 antibody [SP92]
See all BOB1 primary antibodies
DescriptionRabbit monoclonal [SP92] to BOB1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide within Human BOB1 aa 200 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: Q16633
- WB: Ramos cell lysate. IHC-P: Human tonsil tissue.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact email@example.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityProtein A/G purified
Purification notesPurified from TCS by protein A/G.
Our Abpromise guarantee covers the use of ab99891 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration.|
|IHC-P||1/100. Boil tissue section in 10mM Citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.|
FunctionTranscriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.
Tissue specificityB-cell specific.
Involvement in diseaseNote=A chromosomal aberration involving POU2AF1/OBF1 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with BCL6.
Sequence similaritiesBelongs to the POU2AF1 family.
modificationsUbiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
- Information by UniProt
- B cell Oct binding protein 1 antibody
- B cell specific coactivator OBF 1 antibody
- B cell specific coactivator OBF1 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling BOB1 with Purified ab99891 at 1/100 dilution (0.96 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Bond™ Epitope Retrieval Solution 1 (pH 6.0) for 20mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody.
Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Staining of Human BOB1 in a Formalin/PFA-fixed paraffin-embedded section of Human Tonsil using ab99891 at a dilution of 1/100.
Anti-BOB1 antibody [SP92] (ab99891) at 1/100 dilution + lysate prepared from Ramos cells
Observed band size: 35 kDa why is the actual band size different from the predicted?
ab99891 has not yet been referenced specifically in any publications.