Recombinant
RabMAb

Recombinant Anti-BOB1 antibody [SP92] - BSA and Azide free (ab239921)

Overview

  • Product name

    Anti-BOB1 antibody [SP92] - BSA and Azide free
    See all BOB1 primary antibodies
  • Description

    Rabbit monoclonal [SP92] to BOB1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human BOB1 aa 200 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q16633

  • Positive control

    • IHC-P: Human Tonsil, and Human tonsil tissue.
  • General notes

    Ab239921 is the carrier-free version of ab99891. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab239921 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab239921 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.

Boil tissue section in 10mM Citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

 

WB Use at an assay dependent concentration.

Target

  • Function

    Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.
  • Tissue specificity

    B-cell specific.
  • Involvement in disease

    Note=A chromosomal aberration involving POU2AF1/OBF1 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with BCL6.
  • Sequence similarities

    Belongs to the POU2AF1 family.
  • Post-translational
    modifications

    Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • B cell Oct binding protein 1 antibody
    • B cell specific coactivator OBF 1 antibody
    • B cell specific coactivator OBF1 antibody
    • B-cell-specific coactivator OBF-1 antibody
    • BOB 1 antibody
    • BOB-1 antibody
    • OBF 1 antibody
    • OBF1 antibody
    • OBF1_HUMAN antibody
    • OCA B antibody
    • OCA-B antibody
    • OCAB antibody
    • OCT binding factor 1 antibody
    • OCT-binding factor 1 antibody
    • POU class 2 associating factor 1 antibody
    • POU domain class 2 associating factor 1 antibody
    • POU domain class 2-associating factor 1 antibody
    • Pou2af1 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling BOB1 with Purified ab99891 at 1/100 dilution (0.96 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Bond� Epitope Retrieval Solution 1 (pH 6.0) for 20mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab99891)
  • Staining of Human BOB1 in a Formalin/PFA-fixed paraffin-embedded section of Human Tonsil using ab99891 at a dilution of 1/100.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab99891).

References

ab239921 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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