Overview

  • Product name
    Anti-BOG antibody [EPR9905]
    See all BOG primary antibodies
  • Description
    Rabbit monoclonal [EPR9905] to BOG
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide within Human BOG. The exact sequence is proprietary.
    Database link: O75884

  • Positive control
    • HepG2, MCF7, and HeLa cell lysates; kidney tissue; MCF7 and HeLa cells.
  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Previously labelled as RBBP9. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20ºC.
  • Storage buffer
    Constituents: 0.35% Sodium citrate, 0.17% Sodium chloride, 0.03% EDTA, 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR9905
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab157202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      May play a role in the transformation process due to its capacity to confer resistance to the growth-inhibitory effects of TGF-beta1 through interaction with retinoblastoma and the subsequent displacement of E2F-1.
    • Tissue specificity
      Widely expressed. Expressed at higher levels in tumor tissues than in normal tissues.
    • Sequence similarities
      Belongs to the RBBP9 family.
    • Cellular localization
      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • B5T overexpressed gene protein antibody
      • B5T-overexpressed gene protein antibody
      • BOG antibody
      • Bog protein antibody
      • MGC9236 antibody
      • OTTHUMP00000030360 antibody
      • Protein BOG antibody
      • Putative hydrolase RBBP9 antibody
      • RBBP 10 antibody
      • RBBP 9 antibody
      • RBBP-10 antibody
      • RBBP-9 antibody
      • RBBP10 antibody
      • RBBP9 antibody
      • RBBP9_HUMAN antibody
      • Retinoblastoma binding protein 10 antibody
      • Retinoblastoma binding protein 9 antibody
      • Retinoblastoma-binding protein 10 antibody
      • Retinoblastoma-binding protein 9 antibody
      • Retinoma binding protein 9 antibody
      see all

    Images

    • All lanes : Anti-BOG antibody [EPR9905] (ab157202) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : MCF7 cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 21 kDa

    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue, labeling BOG using ab157202 at a 1/100 dilution.

    • Immunofluorescence analysis of MCF7 cells, labeling BOG using ab157202 at a 1/250 dilution.

    • Flow cytometry analysis of permeabilized HeLa cells labeling BOG (red), using ab157202 at a 1/100 dilution, and negative control cells probed with a Rabbit IgG (green)

    References

    ab157202 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab157202.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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