Key features and details
- Mouse monoclonal [OTI4E1] to BOG
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-BOG antibody [OTI4E1]
See all BOG primary antibodies
DescriptionMouse monoclonal [OTI4E1] to BOG
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human BOG aa 1-186. Produced in HEK-293T cells (NP_006597).
MASPSKAVIVPGNGGGDVTTHGWYGWVKKELEKIPGFQCLAKNMPDPITA RESIWLPFMETELHCDEKTIIIGHSSGAIAAMRYAETHRVYAIVLVSAYT SDLGDENERASGYFTRPWQWEKIKANCPYIVQFGSTDDPFLPWKEQQEVA DRLETKLHKFTDCGHFQNTEFHELITVVKSLLKVPA
Database link: O75884
- WB: HEK-293T cell lysate transfected with pCMV6-ENTRY BOG cDNA. ICC/IF: COS-7 cells transiently transfected by pCMV6-ENTRY BOG cDNA.
Clone OTI4E1 (formerly 4E1).
Previously labelled as RBBP9.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 1% BSA
Concentration information loading...
Purification notesPurified from cell culture supernatant by affinity chromatography.
Our Abpromise guarantee covers the use of ab118023 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500. Predicted molecular weight: 21 kDa.|
FunctionMay play a role in the transformation process due to its capacity to confer resistance to the growth-inhibitory effects of TGF-beta1 through interaction with retinoblastoma and the subsequent displacement of E2F-1.
Tissue specificityWidely expressed. Expressed at higher levels in tumor tissues than in normal tissues.
Sequence similaritiesBelongs to the RBBP9 family.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- B5T overexpressed gene protein antibody
- B5T-overexpressed gene protein antibody
- BOG antibody
All lanes : Anti-BOG antibody [OTI4E1] (ab118023) at 1/500 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY RBBP9 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 21 kDa
pCMV6-ENTRY BOG cDNA transfected COS-7 (African green monkey kidney fibroblast-like cell line) cells stained for BOG using ab118023 at a 1/100 dilution in ICC/IF.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab118023 has not yet been referenced specifically in any publications.