Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-Bok antibody [BOK-R1-5-1] (ab233072)

Submit a review Submit a question

Overview

  • Product name

    Anti-Bok antibody [BOK-R1-5-1]
    See all Bok primary antibodies

  • Description

    Rabbit monoclonal [BOK-R1-5-1] to Bok

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Bok aa 1-100. The exact sequence is proprietary.
    Database link: Q9UMX3

  • Positive control

    • WB: MEF, HepG2, MCF7 and C6 whole cell lysate; Human fetal liver, kidney and brain lysate; Mouse brain and liver lysate; Rat brain and spleen lysate. Flow Cytometry: HepG2 and MCF7 cells.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    BOK-R1-5-1

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab233072 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 23 kDa.
Flow Cyt 1/50.

Target

Images

  • Western blot - Anti-Bok antibody [BOK-R1-5-1] (ab233072)
    Western blot - Anti-Bok antibody [BOK-R1-5-1] (ab233072)
    All lanes : Anti-Bok antibody [BOK-R1-5-1] (ab233072) at 1/5000 dilution

    Lane 1 : Wild-type MEF (Mouse embryonic fibroblast cell line) cell lysate
    Lane 2 : Bok knockout MEF cell lysate
    Lane 3 : Wild-type HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
    Lane 4 : Bok knockout HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 23 kDa


    Exposure time: 70 seconds


    Blocking/diluting buffer and concentration: 5% NFDM/TBST.

    The cell lysates were kindly provided by our collaborator Dr. Thomas Kaufmann, University of Bern, Switzerland.

  • Western blot - Anti-Bok antibody [BOK-R1-5-1] (ab233072)
    Western blot - Anti-Bok antibody [BOK-R1-5-1] (ab233072)
    Lanes 1-3 : Anti-Bok antibody [BOK-R1-5-1] (ab233072) at 1/5000 dilution
    Lanes 4-9 : Anti-Bok antibody [BOK-R1-5-1] (ab233072) at 1/1000 dilution

    Lane 1 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg
    Lane 2 : Human fetal liver at 20 µg
    Lane 3 : Human fetal kidney at 20 µg
    Lane 4 : Mouse brain at 10 µg
    Lane 5 : Mouse liver at 10 µg
    Lane 6 : Rat brain at 10 µg
    Lane 7 : Rat spleen at 10 µg
    Lane 8 : C6 (rat glial tumor cell) whole cell lysate at 10 µg
    Lane 9 : Human fetal brain at 10 µg

    Secondary
    Lanes 1-8 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Lane 9 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution

    Predicted band size: 23 kDa



    Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: Lanes 1-3 and 8: 70 seconds; Lanes 4-5: 125 seconds; Lanes 6-7: 37 seconds; Lane 9: 3 minutes.

    The specific band for BOK is observed at 22 kDa; the other bands on the blots are non-specific.

  • Flow Cytometry - Anti-Bok antibody [BOK-R1-5-1] (ab233072)
    Flow Cytometry - Anti-Bok antibody [BOK-R1-5-1] (ab233072)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HepG2 (Human hepatocellular carcinoma epithelial cell) cell line labeling Bok with ab233072 at 1/50 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabelled control (cells incubated with secondary antibody only) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

  • Flow Cytometry - Anti-Bok antibody [BOK-R1-5-1] (ab233072)
    Flow Cytometry - Anti-Bok antibody [BOK-R1-5-1] (ab233072)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized MCF7 (Human breast adenocarcinoma epithelial cell) cells labelling Bok with ab233072 at 1/50 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabelled control (cells incubated with secondary antibody only) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

References

ab233072 has not yet been referenced specifically in any publications.

Publishing research using ab233072? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab233072.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up