Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Bok antibody [BOK-R1-5-1] (ab233072)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [BOK-R1-5-1] to Bok
  • Suitable for: WB, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Bok antibody [BOK-R1-5-1]
    See all Bok primary antibodies

  • Description

    Rabbit monoclonal [BOK-R1-5-1] to Bok

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Bok aa 1-100. The exact sequence is proprietary.
    Database link: Q9UMX3

  • Positive control

    • WB: MEF, HepG2, MCF7 and C6 whole cell lysate; Human fetal liver, kidney and brain lysate; Mouse brain and liver lysate; Rat brain and spleen lysate. Flow Cytometry: HepG2 and MCF7 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    BOK-R1-5-1

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab233072 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 23 kDa.
Flow Cyt 1/50.

Target

Images

  • Western blot - Anti-Bok antibody [BOK-R1-5-1] (ab233072)
    Western blot - Anti-Bok antibody [BOK-R1-5-1] (ab233072)
    All lanes : Anti-Bok antibody [BOK-R1-5-1] (ab233072) at 1/5000 dilution

    Lane 1 : Wild-type MEF (Mouse embryonic fibroblast cell line) cell lysate
    Lane 2 : Bok knockout MEF cell lysate
    Lane 3 : Wild-type HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
    Lane 4 : Bok knockout HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 23 kDa


    Exposure time: 70 seconds


    Blocking/diluting buffer and concentration: 5% NFDM/TBST.

    The cell lysates were kindly provided by our collaborator Dr. Thomas Kaufmann, University of Bern, Switzerland.

  • Western blot - Anti-Bok antibody [BOK-R1-5-1] (ab233072)
    Western blot - Anti-Bok antibody [BOK-R1-5-1] (ab233072)
    Lanes 1-3 : Anti-Bok antibody [BOK-R1-5-1] (ab233072) at 1/5000 dilution
    Lanes 4-9 : Anti-Bok antibody [BOK-R1-5-1] (ab233072) at 1/1000 dilution

    Lane 1 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg
    Lane 2 : Human fetal liver at 20 µg
    Lane 3 : Human fetal kidney at 20 µg
    Lane 4 : Mouse brain at 10 µg
    Lane 5 : Mouse liver at 10 µg
    Lane 6 : Rat brain at 10 µg
    Lane 7 : Rat spleen at 10 µg
    Lane 8 : C6 (rat glial tumor cell) whole cell lysate at 10 µg
    Lane 9 : Human fetal brain at 10 µg

    Secondary
    Lanes 1-8 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Lane 9 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution

    Predicted band size: 23 kDa



    Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: Lanes 1-3 and 8: 70 seconds; Lanes 4-5: 125 seconds; Lanes 6-7: 37 seconds; Lane 9: 3 minutes.

    The specific band for BOK is observed at 22 kDa; the other bands on the blots are non-specific.

  • Flow Cytometry - Anti-Bok antibody [BOK-R1-5-1] (ab233072)
    Flow Cytometry - Anti-Bok antibody [BOK-R1-5-1] (ab233072)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HepG2 (Human hepatocellular carcinoma epithelial cell) cell line labeling Bok with ab233072 at 1/50 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabelled control (cells incubated with secondary antibody only) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

  • Flow Cytometry - Anti-Bok antibody [BOK-R1-5-1] (ab233072)
    Flow Cytometry - Anti-Bok antibody [BOK-R1-5-1] (ab233072)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized MCF7 (Human breast adenocarcinoma epithelial cell) cells labelling Bok with ab233072 at 1/50 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabelled control (cells incubated with secondary antibody only) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (1)

Publishing research using ab233072? Please let us know so that we can cite the reference in this datasheet.

ab233072 has been referenced in 1 publication.

  • Zhang F  et al. Role of B-Cell Lymphoma 2 Ovarian Killer (BOK) in Acute Toxicity of Human Lung Epithelial Cells Caused by Cadmium Chloride. Med Sci Monit 25:5356-5368 (2019). PubMed: 31323016

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