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  1. Link

    bok-antibody-bok-r1-5-1-ab233072.pdf

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Cell Biology Apoptosis Intracellular Bcl2 Family
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Bok antibody [BOK-R1-5-1] (ab233072)

  • Datasheet
  • SDS
  • Certificate of Compliance
Submit a review Submit a question References (3)

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Western blot - Anti-Bok antibody [BOK-R1-5-1] (ab233072)
  • Western blot - Anti-Bok antibody [BOK-R1-5-1] (ab233072)
  • Flow Cytometry (Intracellular) - Anti-Bok antibody [BOK-R1-5-1] (ab233072)
  • Flow Cytometry (Intracellular) - Anti-Bok antibody [BOK-R1-5-1] (ab233072)
  • Anti-Bok antibody [BOK-R1-5-1] (ab233072)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [BOK-R1-5-1] to Bok
  • Suitable for: WB, Flow Cyt (Intra)
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

APC Carrier Free PE

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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
Primary
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Anti-OCRL antibody [EP10256] (ab181039)

View more associated products

Overview

  • Product name

    Anti-Bok antibody [BOK-R1-5-1]
    See all Bok primary antibodies
  • Description

    Rabbit monoclonal [BOK-R1-5-1] to Bok
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt (Intra)more details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: MEF, HepG2, MCF7 and C6 whole cell lysate; Human fetal liver, kidney and brain lysate; Mouse brain and liver lysate; Rat brain and spleen lysate. Flow Cyt (intra): HepG2 and MCF7 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    BOK-R1-5-1
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Bcl2 Family
    • Cell Biology
    • Apoptosis
    • Intracellular
    • p53 Pathway

Associated products

  • Alternative Versions

    • Anti-Bok antibody [BOK-R1-5-1] - BSA and Azide free (ab233700)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Related Products

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)
    • Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab233072 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Predicted molecular weight: 23 kDa.
Flow Cyt (Intra)
1/50.
Notes
WB
1/1000. Predicted molecular weight: 23 kDa.
Flow Cyt (Intra)
1/50.

Target

  • Function

    May play a role in apoptosis.
  • Tissue specificity

    Expressed in brain, liver, appendix and lymphoid tissues.
  • Sequence similarities

    Belongs to the Bcl-2 family.
  • Target information above from: UniProt accession Q9UMX3 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 666 Human
    • Entrez Gene: 51800 Mouse
    • Entrez Gene: 29884 Rat
    • Omim: 605404 Human
    • SwissProt: Q9UMX3 Human
    • SwissProt: O35425 Mouse
    • SwissProt: Q792S6 Rat
    • Unigene: 293753 Human
    • Unigene: 3295 Mouse
    • Unigene: 44461 Rat
    see all
  • Alternative names

    • Bcl 2 related ovarian killer protein antibody
    • Bcl-2-like protein 9 antibody
    • Bcl-2-related ovarian killer protein antibody
    • BCL2 related ovarian killer antibody
    • Bcl2-L-9 antibody
    • BCL2L9 antibody
    • BOK antibody
    • BOK_HUMAN antibody
    • BOKL antibody
    • Hbok antibody
    • MGC4631 antibody
    see all

Images

  • Western blot - Anti-Bok antibody [BOK-R1-5-1] (ab233072)
    Western blot - Anti-Bok antibody [BOK-R1-5-1] (ab233072)
    All lanes : Anti-Bok antibody [BOK-R1-5-1] (ab233072) at 1/5000 dilution

    Lane 1 : Wild-type MEF (Mouse embryonic fibroblast cell line) cell lysate
    Lane 2 : Bok knockout MEF cell lysate
    Lane 3 : Wild-type HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
    Lane 4 : Bok knockout HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 23 kDa


    Exposure time: 70 seconds


    Blocking/diluting buffer and concentration: 5% NFDM/TBST.

    The cell lysates were kindly provided by our collaborator Dr. Thomas Kaufmann, University of Bern, Switzerland.

  • Western blot - Anti-Bok antibody [BOK-R1-5-1] (ab233072)
    Western blot - Anti-Bok antibody [BOK-R1-5-1] (ab233072)
    Lanes 1-3 : Anti-Bok antibody [BOK-R1-5-1] (ab233072) at 1/5000 dilution
    Lanes 4-9 : Anti-Bok antibody [BOK-R1-5-1] (ab233072) at 1/1000 dilution

    Lane 1 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg
    Lane 2 : Human fetal liver at 20 µg
    Lane 3 : Human fetal kidney at 20 µg
    Lane 4 : Mouse brain at 10 µg
    Lane 5 : Mouse liver at 10 µg
    Lane 6 : Rat brain at 10 µg
    Lane 7 : Rat spleen at 10 µg
    Lane 8 : C6 (rat glial tumor cell) whole cell lysate at 10 µg
    Lane 9 : Human fetal brain at 10 µg

    Secondary
    Lanes 1-8 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Lane 9 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution

    Predicted band size: 23 kDa



    Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: Lanes 1-3 and 8: 70 seconds; Lanes 4-5: 125 seconds; Lanes 6-7: 37 seconds; Lane 9: 3 minutes.

    The specific band for BOK is observed at 22 kDa; the other bands on the blots are non-specific.

  • Flow Cytometry (Intracellular) - Anti-Bok antibody [BOK-R1-5-1] (ab233072)
    Flow Cytometry (Intracellular) - Anti-Bok antibody [BOK-R1-5-1] (ab233072)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HepG2 (Human hepatocellular carcinoma epithelial cell) cell line labeling Bok with ab233072 at 1/50 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabelled control (cellsincubated with secondary antibody only) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody. 

     

     

  • Flow Cytometry (Intracellular) - Anti-Bok antibody [BOK-R1-5-1] (ab233072)
    Flow Cytometry (Intracellular) - Anti-Bok antibody [BOK-R1-5-1] (ab233072)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized MCF7 (Human breast adenocarcinoma epithelial cell) cells labelling Bok with ab233072 at 1/50 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabelled control (cellsincubated with secondary antibody only) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody. 

     

     

  • Anti-Bok antibody [BOK-R1-5-1] (ab233072)
    Anti-Bok antibody [BOK-R1-5-1] (ab233072)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (3)

Publishing research using ab233072? Please let us know so that we can cite the reference in this datasheet.

ab233072 has been referenced in 3 publications.

  • Carpio MA  et al. BOK controls apoptosis by Ca2+ transfer through ER-mitochondrial contact sites. Cell Rep 34:108827 (2021). PubMed: 33691099
  • Takahashi J  et al. Interleukin-22 Directly Activates Myocardial STAT3 (Signal Transducer and Activator of Transcription-3) Signaling Pathway and Prevents Myocardial Ischemia Reperfusion Injury. J Am Heart Assoc 9:e014814 (2020). PubMed: 32301368
  • Zhang F  et al. Role of B-Cell Lymphoma 2 Ovarian Killer (BOK) in Acute Toxicity of Human Lung Epithelial Cells Caused by Cadmium Chloride. Med Sci Monit 25:5356-5368 (2019). PubMed: 31323016

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