Product nameAnti-Bok antibody [BOK-R1-5-1]
See all Bok primary antibodies
DescriptionRabbit monoclonal [BOK-R1-5-1] to Bok
Tested applicationsSuitable for: WB, Flow Cytmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Bok aa 1-100. The exact sequence is proprietary.
Database link: Q9UMX3
- WB: MEF, HepG2, MCF7 and C6 whole cell lysate; Human fetal liver, kidney and brain lysate; Mouse brain and liver lysate; Rat brain and spleen lysate. Flow Cytometry: HepG2 and MCF7 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab233072 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 23 kDa.|
FunctionMay play a role in apoptosis.
Tissue specificityExpressed in brain, liver, appendix and lymphoid tissues.
Sequence similaritiesBelongs to the Bcl-2 family.
- Information by UniProt
- Bcl 2 related ovarian killer protein antibody
- Bcl-2-like protein 9 antibody
- Bcl-2-related ovarian killer protein antibody
All lanes : Anti-Bok antibody [BOK-R1-5-1] (ab233072) at 1/5000 dilution
Lane 1 : Wild-type MEF (Mouse embryonic fibroblast cell line) cell lysate
Lane 2 : Bok knockout MEF cell lysate
Lane 3 : Wild-type HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 4 : Bok knockout HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 23 kDa
Exposure time: 70 seconds
Blocking/diluting buffer and concentration: 5% NFDM/TBST.
The cell lysates were kindly provided by our collaborator Dr. Thomas Kaufmann, University of Bern, Switzerland.
Lanes 1-3 : Anti-Bok antibody [BOK-R1-5-1] (ab233072) at 1/5000 dilution
Lanes 4-9 : Anti-Bok antibody [BOK-R1-5-1] (ab233072) at 1/1000 dilution
Lane 1 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2 : Human fetal liver at 20 µg
Lane 3 : Human fetal kidney at 20 µg
Lane 4 : Mouse brain at 10 µg
Lane 5 : Mouse liver at 10 µg
Lane 6 : Rat brain at 10 µg
Lane 7 : Rat spleen at 10 µg
Lane 8 : C6 (rat glial tumor cell) whole cell lysate at 10 µg
Lane 9 : Human fetal brain at 10 µg
Lanes 1-8 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lane 9 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution
Predicted band size: 23 kDa
Blocking/Diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: Lanes 1-3 and 8: 70 seconds; Lanes 4-5: 125 seconds; Lanes 6-7: 37 seconds; Lane 9: 3 minutes.
The specific band for BOK is observed at 22 kDa; the other bands on the blots are non-specific.
Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HepG2 (Human hepatocellular carcinoma epithelial cell) cell line labeling Bok with ab233072 at 1/50 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabelled control (cells incubated with secondary antibody only) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.
Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized MCF7 (Human breast adenocarcinoma epithelial cell) cells labelling Bok with ab233072 at 1/50 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabelled control (cells incubated with secondary antibody only) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.
ab233072 has not yet been referenced specifically in any publications.