Recombinant Anti-Bok antibody [BOK-R1-5-1] (ab233072)
![Western blot - Anti-Bok antibody [BOK-R1-5-1] (ab233072)](https://www.abcam.com/ps/products/233/ab233072/Images/ab233072-306854-anti-bok-antibody-bok-r1-5-1-western-blot.jpg "Western blot - Anti-Bok antibody [BOK-R1-5-1] (ab233072)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BOK-R1-5-1] to Bok
- Suitable for: WB, Flow Cyt (Intra)
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Bok antibody [BOK-R1-5-1]
See all Bok primary antibodies -
Description
Rabbit monoclonal [BOK-R1-5-1] to Bok -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: MEF, HepG2, MCF7 and C6 whole cell lysate; Human fetal liver, kidney and brain lysate; Mouse brain and liver lysate; Rat brain and spleen lysate. Flow Cyt (intra): HepG2 and MCF7 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
BOK-R1-5-1 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233072 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 23 kDa.
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| Flow Cyt (Intra) |
1/50.
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| Notes |
|---|
|
WB
1/1000. Predicted molecular weight: 23 kDa. |
|
Flow Cyt (Intra)
1/50. |
Target
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Function
May play a role in apoptosis. -
Tissue specificity
Expressed in brain, liver, appendix and lymphoid tissues. -
Sequence similarities
Belongs to the Bcl-2 family. - Information by UniProt
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Database links
- Entrez Gene: 666 Human
- Entrez Gene: 51800 Mouse
- Entrez Gene: 29884 Rat
- Omim: 605404 Human
- SwissProt: Q9UMX3 Human
- SwissProt: O35425 Mouse
- SwissProt: Q792S6 Rat
- Unigene: 293753 Human
see all -
Alternative names
- Bcl 2 related ovarian killer protein antibody
- Bcl-2-like protein 9 antibody
- Bcl-2-related ovarian killer protein antibody
see all
Images
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Western blot - Anti-Bok antibody [BOK-R1-5-1] (ab233072)All lanes : Anti-Bok antibody [BOK-R1-5-1] (ab233072) at 1/5000 dilution
Lane 1 : Wild-type MEF (Mouse embryonic fibroblast cell line) cell lysate
Lane 2 : Bok knockout MEF cell lysate
Lane 3 : Wild-type HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 4 : Bok knockout HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 23 kDa
Exposure time: 70 secondsBlocking/diluting buffer and concentration: 5% NFDM/TBST.
The cell lysates were kindly provided by our collaborator Dr. Thomas Kaufmann, University of Bern, Switzerland.
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![Western blot - Anti-Bok antibody [BOK-R1-5-1] (ab233072)](https://www.abcam.com/ps/products/233/ab233072/Images/ab233072-306855-anti-bok-antibody-bok-r1-5-1-western-blot.jpg)
Western blot - Anti-Bok antibody [BOK-R1-5-1] (ab233072)Lanes 1-3 : Anti-Bok antibody [BOK-R1-5-1] (ab233072) at 1/5000 dilution
Lanes 4-9 : Anti-Bok antibody [BOK-R1-5-1] (ab233072) at 1/1000 dilution
Lane 1 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2 : Human fetal liver at 20 µg
Lane 3 : Human fetal kidney at 20 µg
Lane 4 : Mouse brain at 10 µg
Lane 5 : Mouse liver at 10 µg
Lane 6 : Rat brain at 10 µg
Lane 7 : Rat spleen at 10 µg
Lane 8 : C6 (rat glial tumor cell) whole cell lysate at 10 µg
Lane 9 : Human fetal brain at 10 µg
Secondary
Lanes 1-8 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lane 9 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution
Predicted band size: 23 kDaBlocking/Diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: Lanes 1-3 and 8: 70 seconds; Lanes 4-5: 125 seconds; Lanes 6-7: 37 seconds; Lane 9: 3 minutes.
The specific band for BOK is observed at 22 kDa; the other bands on the blots are non-specific.
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![Flow Cytometry (Intracellular) - Anti-Bok antibody [BOK-R1-5-1] (ab233072)](https://www.abcam.com/ps/products/233/ab233072/Images/ab233072-306856-anti-bok-antibody-bok-r1-5-1-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Bok antibody [BOK-R1-5-1] (ab233072)Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HepG2 (Human hepatocellular carcinoma epithelial cell) cell line labeling Bok with ab233072 at 1/50 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabelled control (cellsincubated with secondary antibody only) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.
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![Flow Cytometry (Intracellular) - Anti-Bok antibody [BOK-R1-5-1] (ab233072)](https://www.abcam.com/ps/products/233/ab233072/Images/ab233072-306857-anti-bok-antibody-bok-r1-5-1-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Bok antibody [BOK-R1-5-1] (ab233072)Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized MCF7 (Human breast adenocarcinoma epithelial cell) cells labelling Bok with ab233072 at 1/50 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabelled control (cellsincubated with secondary antibody only) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.
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![Anti-Bok antibody [BOK-R1-5-1] (ab233072)](https://www.abcam.com/ps/products/233/ab233072/Images/ab233072-5-benefits-of-recombinant-antibodies.png)
Anti-Bok antibody [BOK-R1-5-1] (ab233072)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (3)
ab233072 has been referenced in 3 publications.
- Carpio MA et al. BOK controls apoptosis by Ca2+ transfer through ER-mitochondrial contact sites. Cell Rep 34:108827 (2021). PubMed: 33691099
- Takahashi J et al. Interleukin-22 Directly Activates Myocardial STAT3 (Signal Transducer and Activator of Transcription-3) Signaling Pathway and Prevents Myocardial Ischemia Reperfusion Injury. J Am Heart Assoc 9:e014814 (2020). PubMed: 32301368
- Zhang F et al. Role of B-Cell Lymphoma 2 Ovarian Killer (BOK) in Acute Toxicity of Human Lung Epithelial Cells Caused by Cadmium Chloride. Med Sci Monit 25:5356-5368 (2019). PubMed: 31323016