Overview

  • Product name

    Anti-Bok antibody [EPR15331]
    See all Bok primary antibodies
  • Description

    Rabbit monoclonal [EPR15331] to Bok
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Recombinant fragment within Human Bok aa 1-150. The exact sequence is proprietary.
    Database link: Q9UMX3

  • Positive control

    • WB: Human fetal brain, fetal kidney and ovary cancer lysate; HeLa and PC-3 cell lysates; Rat spleen lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR15331
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab186745 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
IP 1/30 - 1/50.

Target

Images

  • All lanes : Anti-Bok antibody [EPR15331] (ab186745) at 1/1000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human fetal kidney lysate
    Lane 3 : Human ovary cancer lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 23 kDa
    Observed band size: 23 kDa

  • All lanes : Anti-Bok antibody [EPR15331] (ab186745) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : PC-3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 23 kDa
    Observed band size: 23 kDa

  • Anti-Bok antibody [EPR15331] (ab186745) at 1/1000 dilution + Rat spleen lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 23 kDa
    Observed band size: 23 kDa

  • Western blot analysis of Bok in PC-3 cell lysate immunoprecipitated with ab186745 at 1/50 dilution (Lane1). Lane 2: PBS instead of PC-3 cell lysate.

    Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

References

This product has been referenced in:

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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