Recombinant
RabMAb

Recombinant Anti-Bok antibody [EPR15331] - BSA and Azide free (ab232610)

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Overview

  • Product name

    Anti-Bok antibody [EPR15331] - BSA and Azide free
    See all Bok primary antibodies

  • Description

    Rabbit monoclonal [EPR15331] to Bok - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WBmore details

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Recombinant fragment within Human Bok aa 1-150. The exact sequence is proprietary.
    Database link: Q9UMX3

  • Positive control

    • WB: Human fetal brain, fetal kidney and ovary cancer tissue lysate.

  • General notes

    Ab232610 is the carrier-free version of ab186745. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab232610 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab232610 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 23 kDa.

Target

Images

  • Immunoprecipitation - Anti-Bok antibody [EPR15331] - BSA and Azide free (ab232610)
    Immunoprecipitation - Anti-Bok antibody [EPR15331] - BSA and Azide free (ab232610)

    Western blot analysis of Bok in PC-3 cell lysate immunoprecipitated with ab186745 at 1/50 dilution (Lane1). Lane 2: PBS instead of PC-3 cell lysate.

    Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab186745).

  • Western blot - Anti-Bok antibody [EPR15331] - BSA and Azide free (ab232610)
    Western blot - Anti-Bok antibody [EPR15331] - BSA and Azide free (ab232610)
    All lanes : Anti-Bok antibody [EPR15331] (ab186745) at 1/1000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human fetal kidney lysate
    Lane 3 : Human ovary cancer lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 23 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab186745).

References

ab232610 has not yet been referenced specifically in any publications.

Publishing research using ab232610? Please let us know so that we can cite the reference in this datasheet.

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