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    bok-antibody-epr15331-bsa-and-azide-free-ab232610.pdf

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Cell Biology Apoptosis Intracellular Bcl2 Family
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RecombinantRabMAb

Recombinant Anti-Bok antibody [EPR15331] - BSA and Azide free (ab232610)

  • Datasheet
  • Certificate of Compliance
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Immunoprecipitation - Anti-Bok antibody [EPR15331] - BSA and Azide free (ab232610)
  • Western blot - Anti-Bok antibody [EPR15331] - BSA and Azide free (ab232610)
  • Anti-Bok antibody [EPR15331] - BSA and Azide free (ab232610)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15331] to Bok - BSA and Azide free
  • Suitable for: IP, WB
  • Reacts with: Rat, Human

You may also be interested in

Conjugation
Product image
HRP Conjugation Kit - Lightning-Link® (ab102890)

View more associated products

Overview

  • Product name

    Anti-Bok antibody [EPR15331] - BSA and Azide free
    See all Bok primary antibodies
  • Description

    Rabbit monoclonal [EPR15331] to Bok - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Recombinant fragment within Human Bok aa 1-150. The exact sequence is proprietary.
    Database link: Q9UMX3

  • Positive control

    • WB: Human fetal brain, fetal kidney and ovary cancer tissue lysate.
  • General notes

    Ab232610 is the carrier-free version of ab186745. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab232610 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR15331
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Bcl2 Family
    • Cell Biology
    • Apoptosis
    • Intracellular
    • p53 Pathway

Associated products

  • Alternative Versions

    • Anti-Bok antibody [EPR15331] (ab186745)
  • Conjugation kits

    • HRP Conjugation Kit - Lightning-Link® (ab102890)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)

Applications

Our Abpromise guarantee covers the use of ab232610 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 23 kDa.

Target

  • Function

    May play a role in apoptosis.
  • Tissue specificity

    Expressed in brain, liver, appendix and lymphoid tissues.
  • Sequence similarities

    Belongs to the Bcl-2 family.
  • Target information above from: UniProt accession Q9UMX3 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 666 Human
    • Entrez Gene: 29884 Rat
    • Omim: 605404 Human
    • SwissProt: Q9UMX3 Human
    • SwissProt: Q792S6 Rat
    • Unigene: 293753 Human
    • Unigene: 44461 Rat
    • Alternative names

      • Bcl 2 related ovarian killer protein antibody
      • Bcl-2-like protein 9 antibody
      • Bcl-2-related ovarian killer protein antibody
      • BCL2 related ovarian killer antibody
      • Bcl2-L-9 antibody
      • BCL2L9 antibody
      • BOK antibody
      • BOK_HUMAN antibody
      • BOKL antibody
      • Hbok antibody
      • MGC4631 antibody
      see all

    Images

    • Immunoprecipitation - Anti-Bok antibody [EPR15331] - BSA and Azide free (ab232610)
      Immunoprecipitation - Anti-Bok antibody [EPR15331] - BSA and Azide free (ab232610)

      Western blot analysis of Bok in PC-3 cell lysate immunoprecipitated with ab186745 at 1/50 dilution (Lane1). Lane 2: PBS instead of PC-3 cell lysate.

      Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab186745).

    • Western blot - Anti-Bok antibody [EPR15331] - BSA and Azide free (ab232610)
      Western blot - Anti-Bok antibody [EPR15331] - BSA and Azide free (ab232610)
      All lanes : Anti-Bok antibody [EPR15331] (ab186745) at 1/1000 dilution

      Lane 1 : Human fetal brain lysate
      Lane 2 : Human fetal kidney lysate
      Lane 3 : Human ovary cancer lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 23 kDa



      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab186745).

    • Anti-Bok antibody [EPR15331] - BSA and Azide free (ab232610)
      Anti-Bok antibody [EPR15331] - BSA and Azide free (ab232610)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (0)

    Publishing research using ab232610? Please let us know so that we can cite the reference in this datasheet.

    ab232610 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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