Recombinant Anti-Bok antibody [EPR15331] - BSA and Azide free (ab232610)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15331] to Bok - BSA and Azide free
- Suitable for: IP, WB
- Reacts with: Rat, Human
Overview
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Product name
Anti-Bok antibody [EPR15331] - BSA and Azide free
See all Bok primary antibodies -
Description
Rabbit monoclonal [EPR15331] to Bok - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant fragment within Human Bok aa 1-150. The exact sequence is proprietary.
Database link: Q9UMX3 -
Positive control
- WB: Human fetal brain, fetal kidney and ovary cancer tissue lysate.
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General notes
Ab232610 is the carrier-free version of ab186745. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab232610 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15331 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab232610 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IP | Use at an assay dependent concentration. | |
WB | Use at an assay dependent concentration. Predicted molecular weight: 23 kDa. |
Target
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Function
May play a role in apoptosis. -
Tissue specificity
Expressed in brain, liver, appendix and lymphoid tissues. -
Sequence similarities
Belongs to the Bcl-2 family. - Information by UniProt
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Database links
- Entrez Gene: 666 Human
- Entrez Gene: 29884 Rat
- Omim: 605404 Human
- SwissProt: Q9UMX3 Human
- SwissProt: Q792S6 Rat
- Unigene: 293753 Human
- Unigene: 44461 Rat
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Alternative names
- Bcl 2 related ovarian killer protein antibody
- Bcl-2-like protein 9 antibody
- Bcl-2-related ovarian killer protein antibody
see all
Images
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Western blot analysis of Bok in PC-3 cell lysate immunoprecipitated with ab186745 at 1/50 dilution (Lane1). Lane 2: PBS instead of PC-3 cell lysate.
Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab186745).
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All lanes : Anti-Bok antibody [EPR15331] (ab186745) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal kidney lysate
Lane 3 : Human ovary cancer lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 23 kDaThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab186745).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab232610 has not yet been referenced specifically in any publications.