Product nameAnti-Bone Sialoprotein antibody
See all Bone Sialoprotein primary antibodies
DescriptionRabbit polyclonal to Bone Sialoprotein
Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
Species reactivityReacts with: Human
- HepG2 whole cell lysate (ab7900).
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab33022 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1.25 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 35 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionBinds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Promotes Arg-Gly-Asp-dependent cell attachment.
modificationsN-glycosylated; glycans consist of sialated and core-fucosylated bi-, tri- and tetraantennary chains.
O-glycosylated at eight sites; mucin-type glycans contain Gal, GlcNAc, GalNAc and terminal NeuAc.
Sulfated on either Tyr-313 or Tyr-314.
- Information by UniProt
- BNSP antibody
- Bone sialoprotein 2 antibody
- Bone sialoprotein II antibody
Anti-Bone Sialoprotein antibody (ab33022) at 1.25 µg/ml + HepG2 cell lysate
HRP conjugated anti Rabbit IgG at 1/50000 dilution
Predicted band size: 35 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
IHC image of ab33022 staining in normal human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab33022, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
This product has been referenced in:
- Marturano-Kruik A et al. Biomechanical regulation of drug sensitivity in an engineered model of human tumor. Biomaterials 150:150-161 (2018). Read more (PubMed: 29040875) »
- Villasante A et al. Tissue-Engineered Model of Human Osteolytic Bone Tumor. Tissue Eng Part C Methods 23:98-107 (2017). Read more (PubMed: 28068876) »