• Product name
    Anti-Bone Sialoprotein antibody
    See all Bone Sialoprotein primary antibodies
  • Description
    Rabbit polyclonal to Bone Sialoprotein
  • Host species
  • Tested applications
    Suitable for: ELISA, RIA, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Human Bone Sialoprotein (bone extract)



Our Abpromise guarantee covers the use of ab52128 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 35 kDa.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/100.


  • Function
    Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Promotes Arg-Gly-Asp-dependent cell attachment.
  • Post-translational
    N-glycosylated; glycans consist of sialated and core-fucosylated bi-, tri- and tetraantennary chains.
    O-glycosylated at eight sites; mucin-type glycans contain Gal, GlcNAc, GalNAc and terminal NeuAc.
    Sulfated on either Tyr-313 or Tyr-314.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • BNSP antibody
    • Bone sialoprotein 2 antibody
    • Bone sialoprotein II antibody
    • BSP antibody
    • BSP II antibody
    • BSPII antibody
    • Cell binding sialoprotein antibody
    • Cell-binding sialoprotein antibody
    • IBSP antibody
    • Integrin binding sialoprotein antibody
    • Integrin-binding sialoprotein antibody
    • SIAL_HUMAN antibody
    • SPII antibody
    see all


  • ab52128 (2ug/ml) staining Bone Sialoprotein in human bone using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of Bone sialoprotein in sectretory compartments in the cells of the perichondrium
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.


This product has been referenced in:
  • Bianco D  et al. Alterations of Subchondral Bone Progenitor Cells in Human Knee and Hip Osteoarthritis Lead to a Bone Sclerosis Phenotype. Int J Mol Sci 19:N/A (2018). Read more (PubMed: 29415458) »
  • Liang D  et al. Effects of nicotine on the metabolism and gene expression profile of Sprague-Dawley rat primary osteoblasts. Mol Med Rep 17:8269-8281 (2018). Read more (PubMed: 29658611) »

See all 8 Publications for this product

Customer reviews and Q&As

Thank you very much for your reply.

The replacement vial of ab125227 will ship out with our next delivery to Biogen. Due to Customs restrictions, this replacement will ship out at the full price ($303.30) but I have arranged credit note # ***...

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I am sorry to hear that the results are still not satisfactory, and I appreciate how much time the customer has spenttroubleshooting. It sounds like the antibody is not work as expected, and I would be happ...

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Thank you very much for your reply and for sending the additional information.

We have not tried the microwave method with this antibody, but one of the other methods (steamer or pressure cooker) may work better. I would suggest using the ste...

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Thank you very much for contacting us and letting us know about the customer's trouble with this antibody.

Threemonths in EDTA is not too long for decalcification. Bone can be a difficult tissue to work with, soit's possible that there is a p...

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Abcam has not validated the combination of species/application used in this Abreview.
Immunohistochemistry (Frozen sections)
Mouse Tissue sections (Endochondral bone)
Endochondral bone
Yes - 0.1% Triton
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 19°C

Abcam user community

Verified customer

Submitted Jan 25 2011

Thank you for your inquiry. Unfortunatly we do not have a specific dilution to recommend for these antibodies. I can suggest to try a range of dilutions from 1:100 to 1:3000 and to optimize the dilution according to the results. Too much antibody w...

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